想做一篇治疗方面的meta分析，都说做系统评价最重要的就是查全、查准文献，在做之前，首先面临的就是查文献这一关。鉴于此，首先师兄将从循证医学基于临床方法学(methodology-based retrieval)的文献检索特点说起。一、传统的基于内容的文献检索方法(content-based retrieval)　　医学信息最常用的是MEDLINE和 EMBASE两大数据库，人们在使用这两个数据库时，通常是根据主题，也就是以内容为 ...

养了很久的细胞，有一些经验和体会总结一下，和大家分享一下。关于如何把细胞养的形态很好更漂亮。

1.不同的细胞，喜欢的环境是不一样的。

这个不仅仅是说培养基的不同，还有就是细胞生长的空间密度问题。

有一些细胞是数量多一点比较好生长，生长状态也比较好。这种一般是属于生长速度慢的细胞。譬如内皮细胞。而有一些是细胞是数量少一点细胞状态会生长的比较好，譬如巨噬细胞和某些肿瘤细胞。尤其是巨噬细胞，生长速度非常得快，贴壁速度很快，所以传代时 ...

1，天然培养基血清、组织提取液等血清：主要是小牛血清、胎牛血清营养成分--白蛋白、纤连蛋白、球蛋白、转铁蛋白、蛋白酶抑制剂、生长因子等蛋白质和多肽；40-80mg/ml氨基酸；0.01-1.0vmol/L 尿素； 170-300ug/ml胆固醇、脂肪酸、磷脂等类脂；2-10mg/ml葡萄糖、乳酸、丙酮酸等碳水化合物；1-2mg/ml钙、氯、铁、钾、钠、锌等无机物；014-0.16mol/L胰岛素、甲状腺素等激素；0.1-200nmol/L维生素A、叶 ...

使用SPSS软件进行数据分析文档通过自己论证属实。主成分分析【例子】以全国31个省市的8项经济指标为例，进行主成分分析。第一步：录入或调入数据（图1）。第二步：打开“因子分析”对话框。沿着主菜单的“Analyze→Data Reduction→Factor”的路径（图2）打开因子分析选项框（图3）。图2 打开因子分析对话框的路径图3 因子分析选项框第三步：选项设置。首先，在源变量框中选中需要进行分析的变量，点击右边的箭头符号，将需要的 ...

Cell-Adhesion AssaysDennis F. Kucik and Chuanyue WuSummaryOne of the most important properties of cells that are derived from multicellularorganisms is their ability to adhere to extracellular matrix proteins or other cells. Analysisof cell–extracellular matrix and/or cell ...

PART II：BASIC CELL MIGRATION AND RELATED ASSAYSvol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 3Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse MicroscopyClaire M. Wells and Anne J. RidleySummaryThe directed mig ...

PART II：BASIC CELL MIGRATION AND RELATED ASSAYSvol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 3Wound-Healing AssayLuis G. Rodriguez, Xiaoyang Wu, and Jun-Lin GuanSummaryThe wound-healing assay is simple, inexpensive, and one of the earliest ...

PART II：BASIC CELL MIGRATION AND RELATED ASSAYSBoyden Chamber Assayvol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 2SummaryThe Boyden chamber assay, originally introduced by Boyden for the analysis ofleukocyte chemotaxis, is based on a cha ...

Cell Migration——An Overviewvol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 1SummaryCell migration is an essential process for normal development and homeostasis thatcan also contribute to important pathologies. Not surprisingly, th ...

去年我一直在跟qPCR、逆转录和SYBRGreen QPCR反应做斗争，其中遇到了不少困难，现在已经告别当初的迷茫。当时在我艰难摸索的时候就想，等我都学会了，我一定要写篇帖子，把当初遇到的一些困难和最后是如何解决这些问题的，写出来，一来可以帮助更多像我这样的人，二来也算让自己有点成就感了。其中有一次，RNA提取好了，跑完胶3条带非常漂亮，测了浓度有500ng/ul呢，做完逆转录，满怀信心的做QPCR反应，做完发现Ct值 ...

Setup of a PCR LaboratoryAbstractPCR represents an extremely powerful and central molecular biology method. At the heart of its poweris the exquisite sensitivity offered: single molecule detection in certain contexts. However, with greatpower comes great responsibility. Con ...

Native Promoter Reporters Validate Transcriptional TargetsMethods in Molecular Biology v468. chapter 9Otto Schmalhofer, Simone Spaderna ,and Thomas BrabletzAbstractThe transcriptional activator b-catenin is the key mediator of the canonical Wnt signaling pathway ...

Assaying b-Catenin/TCF Transcription with b-Catenin/TCFTranscription-Based Reporter ConstructsMethods in Molecular Biology v468. chapter 8AbstractTranscription-based reporters have been instrumental in characterizing the Wnt/b-catenin signalingpa ...

Detection of b-Catenin Localization by ImmunohistochemistryMethods in Molecular Biology v468. chapter 7Abstractβ-catenin is a widely expressed 90-kDa protein with dual functions in cell adhesion and Wnt signalling. At the membrane, β-catenin forms complexes with E-cadherin ...

Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in CellsMethods in Molecular Biology v468. chapter 6AbstractThe adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers.APC is a multi-dom ...

Inhibition of Glycogen Synthase Kinase-3Methods in Molecular Biology v468. chapter 5AbstractThere are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt, Hedgehog, and insulin pathways, as well as the act ...

AbstractGlycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action of Wnts. This protein kinaseis ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling oractivation of growth factor receptor tyrosine kinases (e ...

AbstractRecombinant expression of secreted Frizzled-related proteins (sFRPs) in mammalian expression systemsis a convenient source of these proteins for biological studies. Yields of protein vary; screening of clonallines for high expression is usually worthwhile. Hep ...

Wnt proteins and their signaling cascades are involved in a wide variety of developmental processes, andderegulation of this pathway is frequently associated with tumorigenesis. Unlike many other growthfactors, Wnts long eluded biochemical purification, in large part because of their hydrophobic nature,which is imparted by one or more lipid modifications (1 – 3) . Here I describe a complete protocol thatoutlines the purification process for Wnt proteins. While this protocol has not been applied

Embryonic development of multicellular organisms is an incredibly complex process that relies heavily on evolutionarily conserved signalling pathways to provide crucial cell–cell communication. Typically, secreted signalling proteins such as Wnts, BMPs, and Hedgehogs released by one cell population will trigger concentration-dependent responses in other cells located some distance away. In adults, the same signalling pathways orchestrate tissue renewal in organs such as the intestine and ski