怪兽牙好白
GEMO数据库h5单细胞测序,在合并不同样本时用harmony函数一直报错:Error in `.SelectFeatures()`:
! None of the features provided are present in the feature set
Backtrace:
1. scRNA_harmo %>% ...
3. Seurat:::FindVariableFeatures.Seurat(...)
5. Seurat:::FindVariableFeatures.StdAssay(...)
7. SeuratObject:::VariableFeatures.Assay5(...)
9. SeuratObject:::.SelectFeatures.list(...)
用的是R语言。
scRNA_harmo %>% FindVariableFeatures(.,selection.method = "vst", nfeatures = 2000),就是这句代码报错。
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