跪求各位高手，PCR已做了一个月但是没结果
丁香园论坛
1994
我要得到的目的片段的序列如下：
1: BD073420. Utilization of tr...[gi:22619023]
OCUS BD073420 1266 bp DNA linear PAT 27-AUG-2002
DEFINITION Utilization of transcription factor Brn-3a.
ACCESSION BD073420
VERSION BD073420.1 GI:22619023
KEYWORDS JP 2001511344-A/2.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (bases 1 to 1266)
AUTHORS Damien,S.M. and Seymar,L.D.
TITLE Utilization of transcription factor Brn-3a
JOURNAL Patent: JP 2001511344-A 2 14-AUG-2001;
NEUROVEX LTD
COMMENT OS Mus musculus (mouse)
PN JP 2001511344-A/2
PD 14-AUG-2001
PF 27-JUL-1998 JP 2000504246
PR 25-JUL-1997 GB 9715823.2,10-DEC-1997 US 08/988476 PI
SMITH MARTIN DAMIEN,LATCHMAN DAVID SEYMAR
PC C12N15/09,A61K38/17,A61K39/245,A61K48/00,A61P25/00,C07K14/47,
PC C12N7/00,
PC C12N15/00,A61K37/12
CC Utilization of transcription factor Brn-3a
FH Key Location/Qualifiers
FT CDS (1)..(1266).
FEATURES Location/Qualifiers
source 1..1266
/organism="Mus musculus"
/mol_type="genomic DNA"
/db_xref="taxon:10090"
ORIGIN
1 atgatgtcca tgaacagcaa gcagcctcac tttgccatgc atcccaccct ccctgagcac
61 aagtacccgt cgctgcactc cagctccgag gccatccggc gggcctgcct gcccacgccg
121 ccgctgcaga gcaacctctt cgccagcctg gacgagacgc tgctggcgcg ggccgaggcg
181 ctggcggccg tggacatcgc ggtgtcccag ggcaagagcc accctttcaa gccggacgcc
241 acgtaccaca cgatgaatag cgtgccctgc acgtccacgt ccaccgtgcc gctggcgcac
301 caccaccacc accaccacca ccaccaggcg ctcgagcccg gtgacctgct ggaccacatc
361 tcgtcgccgt cgctcgcgct catggccggc gcagggggcg caggcgcggc gggaggcggc
421 ggcggcgccc acgacggccc cgggggcgga ggcggaccgg ggggcggcgg tggcccgggc
481 ggcggcggcc ccgggggtgg cggcggcggc ggcggcccgg ggggcggcgg cggcgccccg
541 ggcggcgggc tcttgggcgg ctcggcgcat ccgcacccgc acatgcacgg cctgggccac
601 ctgtcgcacc ccgcggcggc ggcggccatg aacatgccgt ccgggctgcc gcatcccggg
661 ctcgtggccg cggcggcgca ccacggcgcg gcggcggcag cggcggcggc ggcggcgggg
721 caggtggcgg cggcgtcggc cgcggcggcg gtggtgggcg cggcgggcct ggcgtccatc
781 tgcgactcgg acacggaccc gcgcgagctc gaggcgttcg ccgagcgctt caagcagcgg
841 cgcatcaagc tgggcgtgac gcaggccgac gtgggctcgg cgctggccaa cctcaagatc
901 ccgggcgtgg gctcgctcag ccagagcacc atctgcaggt tcgagtcgct cacgctctcg
961 cacaacaaca tgatcgcgct caagcccatc ctgcaggcgt ggctggagga ggccgagggc
1021 gcgcagcgtg agaaaatgaa caagccggag ctcttcaacg gcggcgagaa gaagcgcaag
1081 cggacttcca tcgccgcgcc cgagaagcgc tccctcgagg cctattttgc cgtacaaccc
1141 cggccctcgt ctgagaagat cgccgccatc gccgagaaac tggacctcaa aaagaacgtg
1201 gtgcgggtgt ggttttgcaa ccagagacag aagcagaagc ggatgaaatt ctctgccact
1261 tactga
//
已经定了三对引物，其中有一对是文献报道的，结果都一样没有，没有，还是没有。小弟现在陷入极度苦闷，真是生不如死，老板还在后面猛催，
跪请各位高手赐教，帮助设计引物，或是出谋划策均可，呈上十二分感谢。
我的e-mail:1695861859@sina.com.cn
andrewgw1210@hotmail.com
1: BD073420. Utilization of tr...[gi:22619023]
OCUS BD073420 1266 bp DNA linear PAT 27-AUG-2002
DEFINITION Utilization of transcription factor Brn-3a.
ACCESSION BD073420
VERSION BD073420.1 GI:22619023
KEYWORDS JP 2001511344-A/2.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (bases 1 to 1266)
AUTHORS Damien,S.M. and Seymar,L.D.
TITLE Utilization of transcription factor Brn-3a
JOURNAL Patent: JP 2001511344-A 2 14-AUG-2001;
NEUROVEX LTD
COMMENT OS Mus musculus (mouse)
PN JP 2001511344-A/2
PD 14-AUG-2001
PF 27-JUL-1998 JP 2000504246
PR 25-JUL-1997 GB 9715823.2,10-DEC-1997 US 08/988476 PI
SMITH MARTIN DAMIEN,LATCHMAN DAVID SEYMAR
PC C12N15/09,A61K38/17,A61K39/245,A61K48/00,A61P25/00,C07K14/47,
PC C12N7/00,
PC C12N15/00,A61K37/12
CC Utilization of transcription factor Brn-3a
FH Key Location/Qualifiers
FT CDS (1)..(1266).
FEATURES Location/Qualifiers
source 1..1266
/organism="Mus musculus"
/mol_type="genomic DNA"
/db_xref="taxon:10090"
ORIGIN
1 atgatgtcca tgaacagcaa gcagcctcac tttgccatgc atcccaccct ccctgagcac
61 aagtacccgt cgctgcactc cagctccgag gccatccggc gggcctgcct gcccacgccg
121 ccgctgcaga gcaacctctt cgccagcctg gacgagacgc tgctggcgcg ggccgaggcg
181 ctggcggccg tggacatcgc ggtgtcccag ggcaagagcc accctttcaa gccggacgcc
241 acgtaccaca cgatgaatag cgtgccctgc acgtccacgt ccaccgtgcc gctggcgcac
301 caccaccacc accaccacca ccaccaggcg ctcgagcccg gtgacctgct ggaccacatc
361 tcgtcgccgt cgctcgcgct catggccggc gcagggggcg caggcgcggc gggaggcggc
421 ggcggcgccc acgacggccc cgggggcgga ggcggaccgg ggggcggcgg tggcccgggc
481 ggcggcggcc ccgggggtgg cggcggcggc ggcggcccgg ggggcggcgg cggcgccccg
541 ggcggcgggc tcttgggcgg ctcggcgcat ccgcacccgc acatgcacgg cctgggccac
601 ctgtcgcacc ccgcggcggc ggcggccatg aacatgccgt ccgggctgcc gcatcccggg
661 ctcgtggccg cggcggcgca ccacggcgcg gcggcggcag cggcggcggc ggcggcgggg
721 caggtggcgg cggcgtcggc cgcggcggcg gtggtgggcg cggcgggcct ggcgtccatc
781 tgcgactcgg acacggaccc gcgcgagctc gaggcgttcg ccgagcgctt caagcagcgg
841 cgcatcaagc tgggcgtgac gcaggccgac gtgggctcgg cgctggccaa cctcaagatc
901 ccgggcgtgg gctcgctcag ccagagcacc atctgcaggt tcgagtcgct cacgctctcg
961 cacaacaaca tgatcgcgct caagcccatc ctgcaggcgt ggctggagga ggccgagggc
1021 gcgcagcgtg agaaaatgaa caagccggag ctcttcaacg gcggcgagaa gaagcgcaag
1081 cggacttcca tcgccgcgcc cgagaagcgc tccctcgagg cctattttgc cgtacaaccc
1141 cggccctcgt ctgagaagat cgccgccatc gccgagaaac tggacctcaa aaagaacgtg
1201 gtgcgggtgt ggttttgcaa ccagagacag aagcagaagc ggatgaaatt ctctgccact
1261 tactga
//
已经定了三对引物，其中有一对是文献报道的，结果都一样没有，没有，还是没有。小弟现在陷入极度苦闷，真是生不如死，老板还在后面猛催，
跪请各位高手赐教，帮助设计引物，或是出谋划策均可，呈上十二分感谢。
我的e-mail:1695861859@sina.com.cn
andrewgw1210@hotmail.com
