组织学一般技术
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The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for viewing.
Staining Manuals
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Fixative Solutions: Each of the following .PDF files contains the procedure plus a sample container label.
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95% Alcoholic Bouin's Fixative
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95% Alcohol Fixative
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B5 Fixative
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Bouin's Fixative
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Formalin Fixative
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Karnovsky's Fixative (glutaraldehyde)
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Hartman's Fixative
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Hollande's Fixative
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Orth's Solution (dichromate fixative)
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Zenker's Fixative
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Stock Solutions: Each of the following .PDF files contains the information regarding the use and preparation of a working solution.
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Acid cleaning solution for glassware
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Bluing solution for hematoxylin stain
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Celloidin slide adhesive
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Chrome alum adhesive
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Glycerin water mounting medium
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H and E acid decolorizing rinse
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Hypo (sodium thiosulfate)
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Lugol's iodine for mercury removal
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Metal embedding mold release
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Paraffin block softening solution
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Miscellaneous Labels
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Staining Procedures: Each of the following .PDF files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents.
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Introduction
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Acid Fast Stain (for mycobacteria)
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Acid Fast Stain (Ziehl-Neelsen, for M. leprae and Nocardia) [contributed by Greg Winn, Specialist Histopathology & Cytology Services, Western Australia]
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Fite's Acid Fast Stain
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Alcian Blue Stain
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Alcian Blue-PAS Stain (PAB)
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Hyaluronidase Digestion for Alcian Blue
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Alizarin Stain for calcium
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Auramine-Rhodamine Stain (fluorescent)
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Bielschowsky Stain (for senile plaques)
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Bile Stain
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Bodian's Stain
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Colloidal Iron Stain
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Congo Red Stain
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Copper Stain
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Elastic van Gieson Stain
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Elastic - Weigert's resorcin-fuchsin method
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Modified Elastic van Gieson Stain
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Fontana-Masson Stain for melanin
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Melanin Bleach
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Fraser Lendrum Stain
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Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)
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Giemsa Stain (for Helicobacter)
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Gram (Modified Brown-Brenn) Stain
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Gridley's Stain for ameba
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Grimelius Argyrophil Stain (Pascual's Method)
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Grocott's Methenamine Silver (GMS) Stain
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Holzer's Glial Fiber Stain
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Hortega's Pineal Stain
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Iron Stain (Prussian blue)
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Iron Stain (Turnbull's blue)
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Jones' Silver Stain
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Luxol Fast Blue (LFB) Stain
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Methyl Green Pyronin (MGP) Stain
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Mucicarmine Stain
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Nissl Stain
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Oil Red O Stain
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Orcein Stain
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Periodic acid-Schiff Stain (PAS)
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Periodic acid-Schiff, digested Stain (PAS-D)
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PTAH Stain
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Reticulin Stain
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Spirochete Stain (Steiner & Steiner method)
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Sudan Black B Stain (for lipochrome)
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Sudan Black B Stain (for fat)
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Trichrome Stain - Masson's method
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Trichrome Stain - microwave method
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Thioflavin S Stain (for amyloid in tissues)
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Modified Thioflavin S Stain (for senile plaques)
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Toluidine Blue Stain (for mast cells)
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Urate Crystal Stain
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VonKossa Stain for calcium
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Safety Procedures
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Bloodborne Pathogens (Exposure Control Plan)
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Working with Chemicals
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Chemical Hygiene Safety
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Chemical Inventory
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Emergency Procedures
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Facilities (engineering controls)
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Safety Equipment (engineering controls)
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Training Records
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Tuberculosis Guidelines