dxy_5cw6crt2
120mJ/cm2交联不到一分钟就结束了,不知道正不正常?
原文:After Southern transferer, fix the transferred DNA on the wet blotting membrane by UV-crosslinking(120mJ) or by baking the membrane at 120℃ for 20 minutes.
土井挞克树
应该不是的,交联120mj的实际应该到不了120mj/cm2
dxy_5cw6crt2
那是怎么换算呀,交连多了会有什么影响吗?
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