蛋白样品制备一、实验试剂与器材1×PBS，细胞裂解液，组织块，上样缓冲液，细胞刮，移液器，EP管，均浆器，离心机，加热器二、实验步骤事先准备好碎冰，干净的均浆器插在冰上。准备好各种枪头、移液器、EP管、切组织用的消过毒的手术刀、干净的玻璃板和事先准备好的裂解液（根据需要加入合适浓度的蛋白酶抑制剂）↓迅速取出低温冻存的组织块，各样品切取合适大小使称重尽量一致↓将组织低温中研碎，然后倒入EP管，加入裂解液冰上裂解。↓组织被充分 ...
1.根据厂家说明安装玻璃板，用1%热琼脂糖（用1X的电泳液配制，普通琼脂）封闭玻璃板三边间隙，以防灌胶时漏胶。2.在三角瓶中配制8%的分离胶20ml，因为目的蛋白分子量较大，因此用8%的分离胶进行电泳检测；GAPDH 用10%分离胶检测。依次加入各成分，每加一种试剂均要混匀。加入TEMED后，立即快速旋动混合物, 并进行下步的操作。分 离 胶成 分 体积（20ml） 8% 10%水 9.3 8.0430%丙烯酰胺 5.3 6.661.5Mtris(Ph8.8) 5.0 5.010%过硫酸胺 0 ...
On-Line Biology Book: Table of ContentsINTRODUCTION: THE NATURE OF SCIENCE AND BIOLOGY (REVISED 3/12/07)CHEMISTRY I: ATOMS AND MOLECULES (REVISED 3/12/07 )CHEMISTRY II: WATER AND ORGANIC MOLECULES (REVISED 3/12/07)CELLS I: ORIGINS (REVISED 3/12/07)CELLS II: CELLULAR ORGANIZATI ...
觉得蛋白组学是未来科研的重要领域，所以关注了一下，请各位战友补充啊！！:I:I:I:I:P:P:P:P蛋白组学概述http://www.dxy.cn/bbs/thread/840484http://www.dxy.cn/bbs/post/view?bid=65&id=2168890&sty=3&keywords=%B5%B0%B0%D7%D6%CA%D7%E9http://www.dxy.cn/bbs/post/view?bid=65&id=20517 ...
免疫细胞化学技术交流Immunochemistry Protocols: ImmunofluorescenceStep 1Rinse cells attached to cover slips twice with PBS, removing liquid by gentle aspiration in this and subsequent steps.Step 2Fixation & permeabilization:Option I:Fix cells with 4% formaldehyde in PBS for 6 min at ro ...
Western Blot相关的试剂和耗材产 品 名 称包 装单 价(RMB)Trypsin-EDTA0.25%,100ml150.00蛋白裂解液（蛋白提取液）10ml60.00PMSF100mM 10ml86.0040% Acr/Bis (37.5:1)100ml100.0040% Acr/Bis (29:1)100ml100.0040% Acr/Bis (19:1)100ml100.00SDS-PAGE蛋白上样缓冲液2x,1ml28.00SDS-PAGE电泳缓冲液10x,500 ...
一本很好的Protein Microarray Technology 的书Front Matter (p i-xvi)Summary | Full Text: PDF (Size: 3733K)Colour Plates (p xvii-xxxii)Summary | Full Text: PDF (Size: 3504K)Chapter 1:Protein Microarrays: From Fundamental Screening to Clinical Diagnostics (p 1-9)Dr. Dev KambhampatiSu ...
的2009年1-3月全文，持续更新（3月11日更新一期）《科学》09年3月06日出版pdf全文http://www.rayfile.com/files/babebccc-0dd3-11de-9021-0019d11a795f/）重新整理一下，方便大家下载3月08日最新更新三期《自然》09年3月05日出版pdf全文http://www.rayfile.com/files/40b4522e-0b25-11de-b280-001422 ...
UNIT 5.7 Overview of Protein Expression in Pichia pastorisDavid R. Higgins11 Invitrogen Corporation, San Diego, CaliforniaJohn E. Coligan, Ben M. Dunn, David W. Speicher, and Paul T. Wingfield (eds.)Current Protocols in Protein ScienceCopyright © 2003 John Wiley & Sons, Inc. All rights rese ...
我准备做凋亡过程中CASPASE 3的变化，用WESTERN检测。我准备买SANTA CRUZ的抗体。有两种，一种是单抗，另一种是多抗。具体如下：1.caspase-3 (E-8): sc-7272SOURCECaspase-3 (E-8) is a mouse monoclonal IgG2a antibodycorresponding to amino acids 1-277 representing the full lengthprecursor form of caspase-3 (also des ...
Contents:Table of ContentsAdhesion Protein ProtocolsPrefaceContentsContributors1— Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules Selective Protocols of Immunizati...1— Introduction2— Materials3— Methods4— NotesReferences2— Epito ...
TCA-DOCFor precipitation of very low protein concentration1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent).2) Vortex and let sit for 30min at 4ºC.3) Add 1/10 of Trichloroacetic acid (TCA) 100% vortex and let sit ON at 4ºC (preparation of 100% TCA: 454ml H2O/kg TCA. Ma ...
注册了半天，终于搞下来了！http://www.bio-rad.com/cmc_upload/Literature/202685/Install_PDQuest_v801_PC.zipThe download version of PDQuest has a free 30 day trial period, during which you do not normally need an HSK. After the trial period expires, you can continue to use PDQuest in Viewer mo ...
通过对90多种真核蛋白在原核中的表达总结出提高可溶表达的一些经验，很有借鉴意义～AbstractBackground: In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins ...
看了结果却不太明白，究竟有没有跨膜区？能不能做原核表达？谢谢TMpred output for unknown Date: Wed Jul 28 16:50:41 Europe/Zurich 2004--------------------------------------------------------------------------------Sequence: MSS...NNS, length: 431Prediction parameters: TM-helix length between 17 and 33---------------------------------------- ...
Publisher: Wiley-VCHNumber Of Pages: 304Publication Date: 2006-01-09Sales Rank: 488335ISBN / ASIN: 3527308199EAN: 9783527308194Binding: HardcoverManufacturer: Wiley-VCHStudio: Wiley-VCHAverage Rating:Total Reviews:--------------------------------------------------------- ...
Glycoprotein Analysis in BiomedicineGlycoprotein Analysis in Biomedicine(Methods in Molecular Biology)Glycoprotein Analysis in Biomedicine brings together a comprehensive range of protocols related to carbohydrate biochemistry. The first half of the manual outl ...
Publisher: CRCNumber Of Pages: 400Publication Date: 2006-02-23Sales Rank: 871877ISBN / ASIN: 157444526XEAN: 9781574445268Binding: HardcoverManufacturer: CRCStudio: CRCAverage Rating:Total Reviews:--------------------------------------------------------------------------------Book D ...
偶然发现的一个pQE30系统表达蛋白纯化案例，帖出来和大家分享。Purification of LF.诱导与表达E. coli SG13009(pREP4) carrying the recombinant plasmid pPG-LF1 was grown at 37°C in Luria broth with 100 μg of ampicillin and 25 μg of kanamycin per ml at 250 rpm.When the A600 reached 1.0, isopropyl-1-thio-β-d-galactopyran ...
SOUTHERN BLOT1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent to the molecular weight markers as a reference.3. Alkali transfer buffer = 0.5M NaOH (20g/lt), 1.5M NaCl (87.66 g/lt). Prepare 1 litre for one gel and an ...
