蛋白质跨膜区分析结果，请帮忙分析一下。谢谢！
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看了结果却不太明白，究竟有没有跨膜区？能不能做原核表达？谢谢
TMpred output for unknown
[ISREC-Server] Date: Wed Jul 28 16:50:41 Europe/Zurich 2004
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Sequence: MSS...NNS, length: 431
Prediction parameters: TM-helix length between 17 and 33
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1.) Possible transmembrane helices
The sequence positions in brackets denominate the core region.
Only scores above 500 are considered significant.
Inside to outside helices : 3 found
from to score center
183 ( 187) 207 ( 203) 153 195
245 ( 248) 267 ( 267) 412 258
321 ( 321) 339 ( 339) 883 330
Outside to inside helices : 4 found
from to score center
183 ( 187) 206 ( 206) 525 195
245 ( 249) 270 ( 266) 241 257
321 ( 323) 339 ( 339) 597 331
359 ( 359) 382 ( 378) 80 369
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2.) Table of correspondences
Here is shown, which of the inside->outside helices correspond to which of the outside->inside helices.
Helices shown in brackets are considered insignificant.
A "+"-symbol indicates a preference of this orientation.
A "++"-symbol indicates a strong preference of this orientation.
inside->outside | outside->inside
( 183- 207 (25) 153 ) | 183- 206 (24) 525 ++
( 245- 267 (23) 412 + ) |( 245- 270 (26) 241 )
321- 339 (19) 883 ++ | 321- 339 (19) 597
|( 359- 382 (24) 80 ++ )
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3.) Suggested models for transmembrane topology
These suggestions are purely speculative and should be used with extreme caution since they are based on the assumption that all transmembrane helices have been found.
In most cases, the Correspondence Table shown above or the prediction plot that is also created should be used for the topology assignment of unknown proteins.
2 possible models considered, only significant TM-segments used
~undefined*_the_models_differ_in_the_number_of_TM~Fhelices_~3_***~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~8gt~J_STRONGLY_prefered_model~I_N~Fterminus_outside~Kbr_~H~M~2~12_strong_transmembrane_helices~E_total_score_~I_1408~Kbr_~H~M~2~1~5_from_to_length_score_orientation~Kbr_~H~M~2~11_183_206_~A24~B_525_o~Fi~Kbr_~H~M~2~12_321_339_~A19~B_883_i~Fo~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~F~8gt~J_alternative_model~Kbr_~H~M~2~11_strong_transmembrane_helices~E_total_score_~I_883~Kbr_~H~M~2~1~5_from_to_length_score_orientation~Kbr_~H~M~2~11_321_339_~A19~B_883_i~Fo~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~Kbr_~H~M~2~1~Kbr_~H~M~2~1You_can_get_the_prediction_graphics_shown_above_in_one_of_the_following_formats~I~Kbr_~H~M~2~1~Kbr_~H~M~2~1GIF~Fformat~Kbr_~H~M~2~1Postscript~Fformat~Kbr_~H~M~2~1numerical_format
TMpred output for unknown
[ISREC-Server] Date: Wed Jul 28 16:50:41 Europe/Zurich 2004
--------------------------------------------------------------------------------
Sequence: MSS...NNS, length: 431
Prediction parameters: TM-helix length between 17 and 33
--------------------------------------------------------------------------------
1.) Possible transmembrane helices
The sequence positions in brackets denominate the core region.
Only scores above 500 are considered significant.
Inside to outside helices : 3 found
from to score center
183 ( 187) 207 ( 203) 153 195
245 ( 248) 267 ( 267) 412 258
321 ( 321) 339 ( 339) 883 330
Outside to inside helices : 4 found
from to score center
183 ( 187) 206 ( 206) 525 195
245 ( 249) 270 ( 266) 241 257
321 ( 323) 339 ( 339) 597 331
359 ( 359) 382 ( 378) 80 369
--------------------------------------------------------------------------------
2.) Table of correspondences
Here is shown, which of the inside->outside helices correspond to which of the outside->inside helices.
Helices shown in brackets are considered insignificant.
A "+"-symbol indicates a preference of this orientation.
A "++"-symbol indicates a strong preference of this orientation.
inside->outside | outside->inside
( 183- 207 (25) 153 ) | 183- 206 (24) 525 ++
( 245- 267 (23) 412 + ) |( 245- 270 (26) 241 )
321- 339 (19) 883 ++ | 321- 339 (19) 597
|( 359- 382 (24) 80 ++ )
--------------------------------------------------------------------------------
3.) Suggested models for transmembrane topology
These suggestions are purely speculative and should be used with extreme caution since they are based on the assumption that all transmembrane helices have been found.
In most cases, the Correspondence Table shown above or the prediction plot that is also created should be used for the topology assignment of unknown proteins.
2 possible models considered, only significant TM-segments used
~undefined*_the_models_differ_in_the_number_of_TM~Fhelices_~3_***~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~8gt~J_STRONGLY_prefered_model~I_N~Fterminus_outside~Kbr_~H~M~2~12_strong_transmembrane_helices~E_total_score_~I_1408~Kbr_~H~M~2~1~5_from_to_length_score_orientation~Kbr_~H~M~2~11_183_206_~A24~B_525_o~Fi~Kbr_~H~M~2~12_321_339_~A19~B_883_i~Fo~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~F~8gt~J_alternative_model~Kbr_~H~M~2~11_strong_transmembrane_helices~E_total_score_~I_883~Kbr_~H~M~2~1~5_from_to_length_score_orientation~Kbr_~H~M~2~11_321_339_~A19~B_883_i~Fo~Kbr_~H~M~2~1~Kbr_~H~M~2~1~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~F~Kbr_~H~M~2~1~Kbr_~H~M~2~1You_can_get_the_prediction_graphics_shown_above_in_one_of_the_following_formats~I~Kbr_~H~M~2~1~Kbr_~H~M~2~1GIF~Fformat~Kbr_~H~M~2~1Postscript~Fformat~Kbr_~H~M~2~1numerical_format
