请教CASPASE 3抗体的选择?急
丁香园论坛
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我准备做凋亡过程中CASPASE 3的变化,用WESTERN检测。我准备买SANTA CRUZ的抗体。有两种,一种是单抗,另一种是多抗。具体如下:
1.caspase-3 (E-8): sc-7272
SOURCE
Caspase-3 (E-8) is a mouse monoclonal IgG2a antibody
corresponding to amino acids 1-277 representing the full length
precursor form of caspase-3 (also designated CPP32) of human
origin.
SPECIFICITY
Caspase-3 (E-8) is recommended for the detection of caspase-
(also designated CPP32) of human origin by Western blotting,
immunoprecipitation, immunohistochemistry and FCM.
2.caspase-3 (H-277): sc-7148
SOURCE
Caspase-3 (H-277) is a rabbit polyclonal antibody raised against a
recombinant protein corresponding to amino acids 1-277
representing the full length precursor form of caspase-3 of human
origin.
SPECIFICITY
Caspase-3 (H-277) is recommended for the detection of p11, p17
and p20 subunits and the full length precursor of caspase-3 (also
designated CPP32) of mouse, rat and human origin by Western
blotting, immunoprecipitation and immunohistochemistry; may
cross-react with other cystein proteases.
希望各位帮忙!!我用的是细胞系,来源是人的。
1.caspase-3 (E-8): sc-7272
SOURCE
Caspase-3 (E-8) is a mouse monoclonal IgG2a antibody
corresponding to amino acids 1-277 representing the full length
precursor form of caspase-3 (also designated CPP32) of human
origin.
SPECIFICITY
Caspase-3 (E-8) is recommended for the detection of caspase-
(also designated CPP32) of human origin by Western blotting,
immunoprecipitation, immunohistochemistry and FCM.
2.caspase-3 (H-277): sc-7148
SOURCE
Caspase-3 (H-277) is a rabbit polyclonal antibody raised against a
recombinant protein corresponding to amino acids 1-277
representing the full length precursor form of caspase-3 of human
origin.
SPECIFICITY
Caspase-3 (H-277) is recommended for the detection of p11, p17
and p20 subunits and the full length precursor of caspase-3 (also
designated CPP32) of mouse, rat and human origin by Western
blotting, immunoprecipitation and immunohistochemistry; may
cross-react with other cystein proteases.
希望各位帮忙!!我用的是细胞系,来源是人的。