【分享】Glycoprotein Analysis in Biomedicine

Glycoprotein Analysis in Biomedicine

Glycoprotein Analysis in Biomedicine
(Methods in Molecular Biology)

Glycoprotein Analysis in Biomedicine brings together a comprehensive range of protocols related to carbohydrate biochemistry. The first half of the manual outlines physiochemical (MS and NMR), chemical, and chromatographic techniques for defining the structures and diversity of oligosaccharide sequences. The second half describes more biological and immunological approaches used to detect changes in glycosylation patterns in disease.

Contents:

1. A General Strategy for Glycoprotein Oligosaccharide Analysis
Elizabeth Hounsell
pp. 1-16

2. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography
Kazuo Yamamoto; Tsutomu Tsuji; Toshiaki Osawa
pp. 17-34

3. Sequence and Linkage Analysis of N- and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry
Bo Nilsson
pp. 35-46

4. Gas Chromatography and Gas Chromatography–Mass Spectrometry of Glycoprotein Oligosaccharides
Gunnar Hansson; Hasse Karlsson
pp. 47-54

5. Microscale Sequencing of N-Linked Oligosaccharides of Glycoproteins Using Hydrazinolysis, Bio-Gel P-4, and Sequential Exoglycosidase Digestion
Tsuguo Mizuochi
pp. 55-68

6. Analysis of Sugar Chains by Pyridylamination
Sumihiro Hase
pp. 69-80

7. Analysis of Carbohydrates in Glycoproteins by High-Performance Liquid Chromatography and High-Performance Capillary Electrophoresis
Kazuaki Kakehi; Susumu Honda
pp. 81-98

8. The Microanalysis of Glycosyl-Phosphatidylinositol Glycans
M. Güther; Michael Ferguson
pp. 99-118

9. Analysis of Bacterial Glycoproteins
Johann Lechner; Felix Wieland
pp. 119-130

10. Isolation and Fractionation of Glycoprotein Glycans in Small Amounts
Rudolf Geyer; Hildegard Geyer
pp. 131-142

http://rapidshare.de/files/34068465/014-Glycoprotein_Analysis_in_Biomedicine.pdf