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(共享)免疫荧光细胞化学的染色方法

一、标本制作  可制作涂片、印片、细胞单层培养物、组织切片,经适当固定或不固定,作免疫荧光染色用。  二、荧光抗体染色方法  (一)直接法  1.染色 切片经固定后,滴加经稀释至染色效价如1:8或1:16的荧光抗体(如兔抗人γ-球蛋白荧光抗体或兔抗人IgG或IgA荧光抗体等),在室温或37℃染色30min,切片置入能保持潮湿的染色盒内,防止干燥。  2.洗片  倾去存留的荧光抗体,将切片浸入pH7.4或pH7.2PBS中洗两次,搅拌,每次5min,再用蒸馏水洗1m ...

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【共享】Western blot 免疫印迹标准流程(完整版)

这种技术是把电泳分离的组分从凝胶转移到一种固相支持体,并以针对特定氨基酸序列的特异性试剂作为探针检测之。Western使用的探针是抗体,它与附着于固相支持体的靶蛋白所呈现的抗原表位发生特异性反应。这种技术的作用是对非放射性标记蛋白组成的复杂混合物中的某些特异蛋白进行鉴别和鉴定。仪器:高压锅、玻璃匀浆器、高速离心机、分光光度仪、—20℃低温冰箱、垂直板电泳转移装置、恒温水浴摇床、多用脱色摇床。试剂:单去污剂裂解液、0 ...

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【共享】Novo Nordisk 委托丁香园招聘免疫学高级人才

Novo Nordisk China R&D center 委托丁香园网站招聘3位免疫学相关高级人才,待遇优厚,职位详情请参看以下说明,有意者请尽快发送简历至zj@dxyer.com丁香园负责对报名人进行初筛,并将合格简历提交给Novo Nordisk China R&D center 。岗位介绍:Scientist/Senior Scientist/Research DirectorNovo Nordisk China R&D centerZhongguancun Life S ...

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(交流)细胞ELISA

Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x 105 cells/ml for fibroblast cells5. ...

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【共享】书籍:Immunoassays: A Practical Approach (Practical Approach Series)

Immunoassays: A Practical Approach (Practical Approach Series)Author: James P. Gosling (Editor)Publisher: Oxford University Press(UK)Publication Date: 2000-07-15Number Of Pages: 304Format: dvjuSize: 5.94MBSummary:Immunoassays is the first practical volume desi ...

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【资源】Flow Cytometry Protocols (Methods in Molecular Biology)

Review"This is a valuable addition to the literature and will be of benefit to research scientists and clinicians whether they be experienced or new to the methodology." - Cell Biochemistry and FunctionFrom Reviews of the First Edition..."...a well and precisely written collection of protoc ...

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【进展|热点】BLOOD: 雌激素受体通过上调IRF4促进DC的分化

Estrogen receptor signaling promotes dendritic cell differentiation by increasing expression of the transcription factor IRF4Blood, 14 January 2010, Vol. 115, No. 2, pp. 238-246.During inflammation, elevated granulocyte macrophage–colony-stimulating factor (GM-CSF) d ...

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【公告】2006年1月份无人应助贴

1月份ELISA讨论区http://www.dxy.cn/bbs/post/view?bid=68&id=5282143&sty=1&tpg=4&age=0http://www.dxy.cn/bbs/post/view?bid=68&id=5285016&sty=1&tpg=3&age=0http://www.dxy.cn/bbs/post/view?bid=68&id=5408009&sty=1&tpg=2&age=0http://www.dxy.cn/ ...

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Monoclonal Antibody Production Protocol

(1)Cell Fusion and SelectionSolutions:-IMDM-IMDM complete + 15% MCM.-PBS-PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268-Aminopterin: Sigma, A 5159 (50X )Prepare a T-150 flask with 170ml of IMDM complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells.Isolation of ...

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(请教)血清转化是什么意思?

原文Although some asymptomatic and mild infections have been documented, they seem to be uncommon and do not appear to contribute to the extension of the chain of infection among humans. For example, a recent mild infection with SARS-CoV acquired in the laboratory was not transmitted to any contac ...

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B细胞抗原肽表位预测经验,欢迎大家评论

Rules for antigenic peptide selection by wxscmu1.Use more than one peptide2.Use 15-20 AA peptide3.Avoid signal peptide, transmembrane region. Avoid conserved region among both orthologs and paralogs (Do BLAST).4.Use the N- and C-terminal sequences if they are hydrophilic and if a suit ...

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【整理】ELISPOT技术帖子整理

最近要做ELISOPT,把园子里关于ELISOPT的帖子总结了一下,希望对要做ELISPOT的站友有所帮助Elispot技术讨论http://www.dxy.cn/bbs/actions/archive/post/2713205_1.html关于鱼类抗体产生细胞的ELISPOThttp://www.dxy.cn/bbs/actions/archive/post/7762366_1.html做ELISPOT的时候,把PBMC ...

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【资源】Immunopharmacology 2008.PDF

Immunopharmacology 2008.PDFSpringer | 2008-10-06 | ISBN: 0387779752 | 266 pages | PDF | 2,15 MBDuring the past decades, with the introduction of the recombinant DNA, hybridoma and transgenic technologies there has been an exponential evolution in understanding the pathogenesis, dia ...

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【进展|热点】Nature Immunology 聚焦免疫系统

Control systems and decision making for antibody productionChristopher C Goodnow, Carola G Vinuesa, Katrina L Randall, Fabienne Mackay & Robert BrinkThis paper synthesizes recent progress toward understanding the integrated control systems and fail-safes that guide the qual ...

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【共享】书籍:Monoclonal Antibodies-Practical Approach

Monoclonal Antibodies: A Practical Approachby Philip S., Ed. Shepherd (Author)Publisher: Oxford University Press; 1 editionLanguage: EnglishISBN-10: 0199637237ISBN-13: 978-0199637232Book DescriptionMonoclonal Antibodies: A Practical Approach covers the prep ...

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【信息】免疫学圣经7 已经出版了。

Fundamental Immunology 7OverviewFundamental ImmunologySeventh EditionThis standard-setting textbook has defined the field of immunology since 1984, and is now in its Seventh Edition continuing to deliver the detailed, authoritative, and timely coverage readers expec ...

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(资料)荧光物质波长

【资料】荧光物质波长Fluorochrome Excitation Wavelength Emission WavelengthAcid Fuchsin 540 630Acridine Orange(Bound to DNA) 502 526Acridine Red 455-600 560-680Acridine Yellow 470 550Acriflavin 436 520AFA (AcriflavinFeulgen SITSA) 355-425 460Alizarin Complexon 530-560 ...

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【公告】10月份无人应助贴

10月份ELISA讨论区http://www.dxy.cn/bbs/post/view?bid=68&id=4630510&sty=1&tpg=5&age=0http://www.dxy.cn/bbs/post/view?bid=68&id=4617276&sty=1&tpg=5&age=0http://www.dxy.cn/bbs/post/view?bid=68&id=4605198&sty=1&tpg=5&age=0http://www.dxy.cn ...

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【共享】最新《牛津英语词典生物化学与分子生物学》和[Nature][Science]的2009年1-3月全文,持续更新(3月11日更新一期)

的2009年1-3月全文,持续更新(3月11日更新一期)《科学》09年3月06日出版pdf全文http://www.rayfile.com/files/babebccc-0dd3-11de-9021-0019d11a795f/重新整理一下,方便大家下载3月08日最新更新三期《自然》09年3月05日出版pdf全文http://www.rayfile.com/files/40b4522e-0b25-11de-b280-0014221 ...

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【公告】著名免疫学家Eli Sercarz 昨日在美国家中安静离世

http://www.tpims.org/scientists_eli-sercarz.aspSercarz, a native of New York, received his Ph.D. in Immunology at Harvard University in 1960 and had postdoctoral research fellowships at Harvard (1956-1960) and the Massachusetts Institute of Technology (1962-1963). Dr. Serc ...

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