【求助】如何选择C-JUN抗体
丁香园论坛
1796
http://www.univ-bio.com/Products.asp
我在优宁维的网站上查到很多关于C-JUN的抗体，磷酸化又分很多种，不知道如何选择，有知道的战友指点一下，谢谢！
我要准备做WB,IHC.
我在优宁维的网站上查到很多关于C-JUN的抗体，磷酸化又分很多种，不知道如何选择，有知道的战友指点一下，谢谢！
我要准备做WB,IHC.
我觉得如果真想在这家公司购买，咨询该公司就可以了。
其实专业的抗体网站看看就知道应该要了解那些信息。如Abcam、santa cruz和cst等等
最后，提供一个学习的博客，可以看看http://blog.sina.com.cn/biolinkcanton
其实专业的抗体网站看看就知道应该要了解那些信息。如Abcam、santa cruz和cst等等
最后，提供一个学习的博客，可以看看http://blog.sina.com.cn/biolinkcanton
谢谢，咨询了几个人后，我已经定购了CST的。因为挺贵的，怕浪费了。
c-Jun is also phosphorylated at two residues proximal to the major transactivation domain. These residues (Ser 63 and 73) are required to be phosphorylated for efficient transactivation function. The kinases responsible for this modification in vivo are the SAPK/JNKs. These kinases bind with very high affinity to a region in c-Jun termed the delta domain. This region is deleted in v-Jun. v-Jun is not phosphorylated but is transcriptionally active. Thus, binding of inactive SAPK to Jun may represent a mechanism for inhibiting the function of the transcription factor. Once activated, the SAPK will phosphorylate c-Jun and dissociate. Mutation of the phosphorylation sites prevents dissociation thus resulting in inactive c-Jun. v-Jun, on the other hand, cannot bind the kinases and so is constitutively active.
http://kinase.uhnres.utoronto.ca/Map/Jun.html
http://kinase.uhnres.utoronto.ca/Map/Jun.html
chuckdouble wrote:
c-Jun is also phosphorylated at two residues proximal to the major transactivation domain. These residues (Ser 63 and 73) are required to be phosphorylated for efficient transactivation function. The kinases responsible for this modification in vivo are the SAPK/JNKs. These kinases bind with very high affinity to a region in c-Jun termed the delta domain. This region is deleted in v-Jun. v-Jun is not phosphorylated but is transcriptionally active. Thus, binding of inactive SAPK to Jun may represent a mechanism for inhibiting the function of the transcription factor. Once activated, the SAPK will phosphorylate c-Jun and dissociate. Mutation of the phosphorylation sites prevents dissociation thus resulting in inactive c-Jun. v-Jun, on the other hand, cannot bind the kinases and so is constitutively active.
http://kinase.uhnres.utoronto.ca/Map/Jun.html
挺难理解的，我要查查资料先。
只能投票感谢二位的帮助。
临床医生做试验，真是难啊。再次谢谢。
临床医生做试验，真是难啊。再次谢谢。
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