luciferase检测的标准品问题

荧光素酶作为一种报告基因要在检测时比较其相对发光强度值，就要在转染时引入另外一个报告基因进行转染条件、效率的标准化归一。

Promega公司在这方面有双荧光素酶检测试剂盒，就是转染时同时转入两个质粒，包括萤火虫荧光素酶催化的反应和海肾（一种来自海洋的生物发光动物──三色堇）荧光素酶催化的反应。下面是说明书的一段原话：

Firefly and Renilla luciferases，because of their distinct evolutionary origins，

have dissimilar enzyme structures and substrate requirements.These differences

make it possible to selectively discriminate between their respective

bioluminescent reactions.Thus，using the DLR™； Assay Systems，the

luminescence from the firefly luciferase reaction may be quenched while

simultaneously activating the luminescent reaction of Renilla luciferase.