请各位大侠帮忙看一下，急等××××××，感激不尽
丁香园论坛
445
某基因的序列如下，我设计了一对引物，5 TCGGTTTTCACTGGTTCTCATCC 3
3 T CGCACTCCTTTCTCTCGCAATG 5
想扩增的目的片断是3629－4799 （1170 bp，）不知道能否，请大侠们帮忙看一下，
3601 ttttttaatt tcaggatatg gagcttcttc ggttttcact ggttctcatc cagtcctggc
3661 tgaccccagt gcaataccta agcaaggtgt ttacaaacaa cctggttttt ggcacctcag
3721 acagagtgtt tgaaaaacta aaggacctag aagaagggat ccaagctctg atgagggtaa
3781 gttgcagtag gtagcatgat tacggagtaa caatctccta aacacagggc actgagctct
3841 cagggtcttc tacaggatcc aaagaccatg agaagtctcc ccaccttcca ctccaaaaaa
3901 cattggcatc ctttgaactt tgcttacttt aacatagtca catcacaaca ctccactgca
3961 cacaattcat cactgggagc atgagaaaac ttgtaccaga tgttaacagc actgccataa
4021 cctgcatagc agcacaggcc aggaagatca caacagtcta gactcagtct ttacaaaccc
4081 agcaccaaag aatactccat tggttaaact tgctgttaaa cctgatgttt gccctatctc
4141 ccagaaaaca ctttaaagtg aagcaaattt tgcagagttc tgcattcctg acttccaaga
4201 ctcaagacat tttataaaac aactcatata agtctgtgta ctgatggaag cttatactct
4261 gatgtttctc tcttatatta catttaaatt aacctagcac gcagacaact gacagctgga
4321 gaatgttctt cagaaagatt acttttggct ttgttatttt aaatgacatt acttattgtc
4381 tgtcaggatc ccatcttaga tttgctcagc atcaccacag ctagagcccc aagacctgtc
4441 tccatgtgat gtcagtctgg acgatgattt tcatcatcta gcacctaaat aactacaaaa
4501 gtcaggcaca gagagtaaac aaagatcttc tgtcctgact gtagtctgta gcatttccag
4561 gttttgctct ccgtttgata taagtttggg caatgggaag cagtacacag taaccaggta
4621 atgatcactc tttagccttt taacaccctg tttactgaac aaaaacggtt ccgttgaccg
4681 aatcagctgg caagcaaagc agggcaggag gagaggcggc ctgcagctcc agatgcacat
4741 ggcttgggtc tggcttcaag ctctggtcac tggaaaagcg tgaggaaaga gagcgttact
4801 gacccctcgc atgacctcct cctctccagt ggctacggcc ggactctgct tccctgcccc
4861 actttttaga gcccctggtg cgagctcagc cccggccctt tccctcctgc aggagctgga
4921 ggaccggagc ccgcggggcc cgcagctcct caagcccacc tacgacaagt tcgacatcca
4981 cctgcgcaac gaggacgccc tgctgaagaa ctacggcctg ctctcctgct tcaagaagga
3 T CGCACTCCTTTCTCTCGCAATG 5
想扩增的目的片断是3629－4799 （1170 bp，）不知道能否，请大侠们帮忙看一下，
3601 ttttttaatt tcaggatatg gagcttcttc ggttttcact ggttctcatc cagtcctggc
3661 tgaccccagt gcaataccta agcaaggtgt ttacaaacaa cctggttttt ggcacctcag
3721 acagagtgtt tgaaaaacta aaggacctag aagaagggat ccaagctctg atgagggtaa
3781 gttgcagtag gtagcatgat tacggagtaa caatctccta aacacagggc actgagctct
3841 cagggtcttc tacaggatcc aaagaccatg agaagtctcc ccaccttcca ctccaaaaaa
3901 cattggcatc ctttgaactt tgcttacttt aacatagtca catcacaaca ctccactgca
3961 cacaattcat cactgggagc atgagaaaac ttgtaccaga tgttaacagc actgccataa
4021 cctgcatagc agcacaggcc aggaagatca caacagtcta gactcagtct ttacaaaccc
4081 agcaccaaag aatactccat tggttaaact tgctgttaaa cctgatgttt gccctatctc
4141 ccagaaaaca ctttaaagtg aagcaaattt tgcagagttc tgcattcctg acttccaaga
4201 ctcaagacat tttataaaac aactcatata agtctgtgta ctgatggaag cttatactct
4261 gatgtttctc tcttatatta catttaaatt aacctagcac gcagacaact gacagctgga
4321 gaatgttctt cagaaagatt acttttggct ttgttatttt aaatgacatt acttattgtc
4381 tgtcaggatc ccatcttaga tttgctcagc atcaccacag ctagagcccc aagacctgtc
4441 tccatgtgat gtcagtctgg acgatgattt tcatcatcta gcacctaaat aactacaaaa
4501 gtcaggcaca gagagtaaac aaagatcttc tgtcctgact gtagtctgta gcatttccag
4561 gttttgctct ccgtttgata taagtttggg caatgggaag cagtacacag taaccaggta
4621 atgatcactc tttagccttt taacaccctg tttactgaac aaaaacggtt ccgttgaccg
4681 aatcagctgg caagcaaagc agggcaggag gagaggcggc ctgcagctcc agatgcacat
4741 ggcttgggtc tggcttcaag ctctggtcac tggaaaagcg tgaggaaaga gagcgttact
4801 gacccctcgc atgacctcct cctctccagt ggctacggcc ggactctgct tccctgcccc
4861 actttttaga gcccctggtg cgagctcagc cccggccctt tccctcctgc aggagctgga
4921 ggaccggagc ccgcggggcc cgcagctcct caagcccacc tacgacaagt tcgacatcca
4981 cctgcgcaac gaggacgccc tgctgaagaa ctacggcctg ctctcctgct tcaagaagga
