【求助】请教这是用的什么方法进行基因分型？
丁香园论坛
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在一篇文章中看到如下一段话，怎么看也没明白怎么测得基因分型，请教下
Activity phenotype method
Rates of POase activity and DZOase activity were measured spectrophotometrically in serum according to the method described by Richter and Furlong (24). Briefly, POase and DZOase activities were measured by continuous spectrophotometric assays using a UV/VIS Ultrospec III spectrophotometer (Pharmacia LKB, Cambridge, UK). The assay buffer for both enzyme activities was the same (0.1 mol/L Tris-HCl, pH8.5, 2 M NaCl and 2 mmol/L CaCl2). The concentrations of paraoxon and diazoxon were 1.2 mmol/L and 1 mmol/L,respectively. Both paraoxon and diazoxon were purchased
from Chem Service (West Chester, PA, USA). POase activity was determined at 258C, whereas DZOase activity was determined at 238C. Both POase and DZOase activities were saltstimulated PON1 activities. The conversion of paraoxon to p-nitrophenol by the hydrolytic activity of POase was monitored at 405 nm. DZOase activity was determined by the rate of diazoxon hydrolysis and the subsequent production of
2-isopropyl-4-methyl-6-hydroxypyrimidine (monitored at 270 nm). All samples were measured as duplicates and the mean value was used for comparative analyses. The activities are reported as mmol/min/L (noted as IU/L). POase and DZOase activity measurements were performed in serum samples stored for up to 12 months at –808C, as PON1 activity has been described to be stable for more than 12 months(25). We tested PON1 activity in batches of sera over a period of up to 2 years. The first significant fall in activity was only detected after 18 months. The intra-assay coefficients of variation were 5.4% and 6.8% for POase and DZOase, respectively.
The inter-assay coefficients of variation were 7.7% and 9.5% for POase and DZOase, respectively. The PON1192 phenotype was predicted after examination of the two-dimensional plot of diazoxon vs. paraoxon hydrolysis rates (Figure 2A) and also by calculating the DZOase/POase activity ratio. PON1192 phenotyping was also performed using the antimode of the histogram of the DZOase/POase activity ratio
(Figure 2B) as proposed by Adkins et al. (26). Individuals with the QQ phenotype have a DZOase/POase activity ratio above 60, individuals with the QR phenotype between 21 and 59, and individuals with the RR phenotype below 20.
Activity phenotype method
Rates of POase activity and DZOase activity were measured spectrophotometrically in serum according to the method described by Richter and Furlong (24). Briefly, POase and DZOase activities were measured by continuous spectrophotometric assays using a UV/VIS Ultrospec III spectrophotometer (Pharmacia LKB, Cambridge, UK). The assay buffer for both enzyme activities was the same (0.1 mol/L Tris-HCl, pH8.5, 2 M NaCl and 2 mmol/L CaCl2). The concentrations of paraoxon and diazoxon were 1.2 mmol/L and 1 mmol/L,respectively. Both paraoxon and diazoxon were purchased
from Chem Service (West Chester, PA, USA). POase activity was determined at 258C, whereas DZOase activity was determined at 238C. Both POase and DZOase activities were saltstimulated PON1 activities. The conversion of paraoxon to p-nitrophenol by the hydrolytic activity of POase was monitored at 405 nm. DZOase activity was determined by the rate of diazoxon hydrolysis and the subsequent production of
2-isopropyl-4-methyl-6-hydroxypyrimidine (monitored at 270 nm). All samples were measured as duplicates and the mean value was used for comparative analyses. The activities are reported as mmol/min/L (noted as IU/L). POase and DZOase activity measurements were performed in serum samples stored for up to 12 months at –808C, as PON1 activity has been described to be stable for more than 12 months(25). We tested PON1 activity in batches of sera over a period of up to 2 years. The first significant fall in activity was only detected after 18 months. The intra-assay coefficients of variation were 5.4% and 6.8% for POase and DZOase, respectively.
The inter-assay coefficients of variation were 7.7% and 9.5% for POase and DZOase, respectively. The PON1192 phenotype was predicted after examination of the two-dimensional plot of diazoxon vs. paraoxon hydrolysis rates (Figure 2A) and also by calculating the DZOase/POase activity ratio. PON1192 phenotyping was also performed using the antimode of the histogram of the DZOase/POase activity ratio
(Figure 2B) as proposed by Adkins et al. (26). Individuals with the QQ phenotype have a DZOase/POase activity ratio above 60, individuals with the QR phenotype between 21 and 59, and individuals with the RR phenotype below 20.
文献里说得很明白：“as proposed by Adkins et al. (26).”。你看看Adkin的这篇文献不就很清楚了么？
freecell wrote:
文献里说得很明白：“as proposed by Adkins et al. (26).”。你看看Adkin的这篇文献不就很清楚了么？
对头！谢啦
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