哪位大虾帮我用Blast验证一下（引物和序列己设计好），我看不太懂！谢谢！
丁香园论坛
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准备做Real time-PCR,目的基因序列（cDNA)和引物见下（PP5.0设计）
我用Blast分析了一下，可是出来的结果不太懂，有哪位大虾解释一下及给出建议。谢谢！
BLAST地址：http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi
结果：见附图
上游：CTCTGGCTGACTTGGCTTGG
下游：GATTTGGTTGCTCTGTTGACTC
原cDNA序列：
atgaccgcgc ggggcgccgc ggggcgctgc ccttcttcga catggatggg ctcccggctg
61 ctgctggtct gtctcctcgt gagcaggagt gttgccgagg tgtcggagca ctgtagccac
121 atgattggga atggacacct acagattttg cagcagttga tcgacagcca aatggagact
181 gcatgcctga tcgaatataa atttgtagac caggaacagc tggacgatcc cgtttgctac
241 ctaaagaagg cctttgtttt ggtacaagtc ataatcgagg aaaccatgcg ctttaaagac
301 aacaccccca acgccaacgc caccgagagg ctacaggaac tctctatgaa actgaacagc
361 tgctttatca aggactataa ggaacagaac gaggcctgtg tccaaactta caaagagagt
421 cctctccggt tgctggagaa aatcaagaat ttctttaacg aaacaaagaa tttccttgaa
481 aaggactgga atatttttag caagaactgc aatgacagct ttgctaaatg ctctagcaga
541 gatgtggtga ccaagcctga ttgcaactgc ctgtacccta aagccacccc tagcagcgac
601 ctggcctctg cctcccctca ccagccccct gccccctcca tggcccctct ggctgacttg
661 gcttgggatg attctcagag gacagaggga agctccctct tgcccagtga tctgcctctt
721 cgcatagagg acccaggcag tgctaagcag cgaccgccca ggagtacctg ccagaccctc
781 gagtcaacag agcaaccaaa tcacgaggac ccacaacctc atccttctgc gggggccccc
841 atccctgggg tggaagacat tattgaatct tcaatgggca ctaactgggt cttagaagaa
901 gcttctggag aggctagtga gggatttttg acccaggaaa ggaagttttc cccctccaat
961 cctgtagggg gcagcatcca ggcagagact gacagaccct gggcccgctc agcatcttct
1021 ccattcccta aattaacaga ggatcaacag ccaacgaata taacagacac cccgttgaca
1081 gaggtgaacc ctatgagacc cactggccag acactgaata atactcctga gaagacggat
1141 ggttcatcta cactgcgcga agaccagcag gagccacgct ctccccattt tgccacactg
1201 aatccccaac gagtcggcaa ctcagccacc ccctatgcta agttactgcc tcccaaaagc
1261 cactcttggg gcattgtact gccccttggg gagctcgagg gcaagaaaag tactagagat
1321 cgaaggagcc ccgcagagct gaaaggagga ccagcaagtg agggggcagc caggcctgtg
1381 gcccaaagta ctagagatcg aaggagcccc gcagagctga aaggaggacc agcaagtgag
1441 ggggcagcca ggcctgtggc ccgttttaat tccattcctt tgaccgacac aggctcttct
1501 attcaggatc cccagacctc tgcgtttgtc ttctgggtgc taggcatcat cctagtcttg
1561 ctggctgtcg ggggcctcct gttctacagt tggaagcgga ggagccatcg agaccctcgg
1621 acattggatt cttctgtggg gcgaccagag ggcagctccc tggcccagga tgaggacaga
1681 caggtggaac tgccagtgta g
我用Blast分析了一下，可是出来的结果不太懂，有哪位大虾解释一下及给出建议。谢谢！
BLAST地址：http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi
结果：见附图
上游：CTCTGGCTGACTTGGCTTGG
下游：GATTTGGTTGCTCTGTTGACTC
原cDNA序列：
atgaccgcgc ggggcgccgc ggggcgctgc ccttcttcga catggatggg ctcccggctg
61 ctgctggtct gtctcctcgt gagcaggagt gttgccgagg tgtcggagca ctgtagccac
121 atgattggga atggacacct acagattttg cagcagttga tcgacagcca aatggagact
181 gcatgcctga tcgaatataa atttgtagac caggaacagc tggacgatcc cgtttgctac
241 ctaaagaagg cctttgtttt ggtacaagtc ataatcgagg aaaccatgcg ctttaaagac
301 aacaccccca acgccaacgc caccgagagg ctacaggaac tctctatgaa actgaacagc
361 tgctttatca aggactataa ggaacagaac gaggcctgtg tccaaactta caaagagagt
421 cctctccggt tgctggagaa aatcaagaat ttctttaacg aaacaaagaa tttccttgaa
481 aaggactgga atatttttag caagaactgc aatgacagct ttgctaaatg ctctagcaga
541 gatgtggtga ccaagcctga ttgcaactgc ctgtacccta aagccacccc tagcagcgac
601 ctggcctctg cctcccctca ccagccccct gccccctcca tggcccctct ggctgacttg
661 gcttgggatg attctcagag gacagaggga agctccctct tgcccagtga tctgcctctt
721 cgcatagagg acccaggcag tgctaagcag cgaccgccca ggagtacctg ccagaccctc
781 gagtcaacag agcaaccaaa tcacgaggac ccacaacctc atccttctgc gggggccccc
841 atccctgggg tggaagacat tattgaatct tcaatgggca ctaactgggt cttagaagaa
901 gcttctggag aggctagtga gggatttttg acccaggaaa ggaagttttc cccctccaat
961 cctgtagggg gcagcatcca ggcagagact gacagaccct gggcccgctc agcatcttct
1021 ccattcccta aattaacaga ggatcaacag ccaacgaata taacagacac cccgttgaca
1081 gaggtgaacc ctatgagacc cactggccag acactgaata atactcctga gaagacggat
1141 ggttcatcta cactgcgcga agaccagcag gagccacgct ctccccattt tgccacactg
1201 aatccccaac gagtcggcaa ctcagccacc ccctatgcta agttactgcc tcccaaaagc
1261 cactcttggg gcattgtact gccccttggg gagctcgagg gcaagaaaag tactagagat
1321 cgaaggagcc ccgcagagct gaaaggagga ccagcaagtg agggggcagc caggcctgtg
1381 gcccaaagta ctagagatcg aaggagcccc gcagagctga aaggaggacc agcaagtgag
1441 ggggcagcca ggcctgtggc ccgttttaat tccattcctt tgaccgacac aggctcttct
1501 attcaggatc cccagacctc tgcgtttgtc ttctgggtgc taggcatcat cctagtcttg
1561 ctggctgtcg ggggcctcct gttctacagt tggaagcgga ggagccatcg agaccctcgg
1621 acattggatt cttctgtggg gcgaccagag ggcagctccc tggcccagga tgaggacaga
1681 caggtggaac tgccagtgta g
