Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying
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The aim of preserving a fungus is to maintain it in a viable state without change to its genetic, physiological, or anatomical
characters. There are numerous methodologies available to preserve a fungus, but the two methods widely used by culture collections
(biological or genetic resource centers) to achieve successful preservation are cryopreservation with liquid nitrogen using
controlled-rate freezing and centrifugal freeze-drying. Generic methods are often used, but specific variations of a method
may be required in order to achieve optimal stability. No single method can be applied to all fungi. More recently, techniques
such as vitrification and encapsulation cryopreservation have been used to preserve recalcitrant fungi. The protocols described
within this chapter have been developed over many years at one of the world’s largest culture collections of filamentous fungi.