Freeze-Drying Fungi Using a Shelf-Freeze Drier
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Lyophilization, the removal of water by freezing and then volatilization at low pressure and temperature, has been employed
as a standard long-term preservation method for many filamentous fungi. The method outlined involves the use of standard shelf
freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority
of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture
collection (http://www.cbs.knaw.nl/
).
