帮忙设计REAL TIM PCR引物，谢谢！

希望各位大侠帮忙设计下CASPASE-3的REAL TIME PCR引物。以下是序列：
1 atggacaaca acgaaacctc cgtggattca aaatccatta ataattttga aacaaagact
61 atccatggaa gcaagtcgat ggactctgga atatatctgg acagcagtta caaaatggat
121 taccctgaaa tgggcttgtg tataataatt aataataaga acttccataa aagcactgga
181 atgtcagctc gcaatggtac cgatgtcgat gcagctaacc tcagagagac attcatggcc
241 ctgaaatacg aagtcaggaa taaaaatgac cttactcgtg aagaaattat ggaattgatg
301 gatagtgttt ctaaggaaga tcacagcaaa aggagcagtt ttgtgtgtgt gattctaagt
361 catggagatg aaggagtaat ttttggaacg aacggacctg tggacctgaa aaaactaact
421 agtttcttca gaggcgacta ctgccggagt ctgactggaa agccgaaact cttcatcatt
481 caggcctgcc gaggtacaga gctggactgc ggtattgaga cagacagtgg aactgacgat
541 gatatggcat gccagaagat accagtggag gccgacttcc tgtatgctta ctctaccgca
601 cccggttact attcctggag aaattcaagg gacgggtcat ggttcatcca gtcactttgc
661 gccatgctga aactgtacgc gcacaagctg gaattcatgc acatcctcac tcgtgttaac
721 cggaaggtgg ccacggaatt tgagtccttc tccctggacg ccactttcca tgcaaagaaa
781 cagatcccgt gtattgtgtc aatgctcaca aaagaactgt acttttatca ctaa