帮忙设计引物

TITLE Organization and structure of the mouse interleukin-2 gene
JOURNAL Nucleic Acids Res. 12 (24), 9323-9331 (1984)
PUBMED 6240025
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from X01772.1.
On or before Dec 17, 2003 this sequence version replaced
gi:38077356, gi:38077337, gi:7110652.
FEATURES Location/Qualifiers
source 1..939
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/chromosome="3"
/map="3 19.2 cM"
gene 1..939
/gene="Il2"
/note="synonym: Il-2"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
CDS 48..557
/gene="Il2"
/note="go_component: extracellular [goid 0005576]
[evidence IEA];
go_component: extracellular space [goid 0005615] [evidence
TAS] [pmid 12466851];
go_function: cytokine activity [goid 0005125] [evidence
IEA];
go_function: interleukin-2 receptor binding [goid 0005134]
[evidence TAS] [pmid 9741021];
go_function: growth factor activity [goid 0008083]
[evidence IEA];
go_function: hormone activity [goid 0005179] [evidence
TAS] [pmid 1830926];
go_process: defense response [goid 0006952] [evidence IMP]
[pmid 7589135];
go_process: inflammatory response [goid 0006954] [evidence
IMP] [pmid 9741021];
go_process: immune response [goid 0006955] [evidence TAS]
[pmid 8402910];
go_process: cellular defense response [goid 0006968]
[evidence TAS] [pmid 8402910];
go_process: cell proliferation [goid 0008283] [evidence
IMP] [pmid 8402910]"
/codon_start=1
/product="interleukin 2"
/protein_id="NP_032392.1"
/db_xref="GI:7110653"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
/translation="MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQ
QQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRH
VLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFC
QSIISTSPQ"
misc_feature 48..551
/gene="Il2"
/note="IL2; Region: Interleukin-2 family"
/db_xref="CDD:166"
sig_peptide 48..107
/gene="Il2"
/product="signal peptide (aa -21 to -1)"
mat_peptide 108..554
/gene="Il2"
/product="interleukin-2 (aa 1-133)"
repeat_region 150..185
/note="12 tandem repeats of CAG"
ORIGIN
1 atcacccttg ctaatcactc ctcacagtga cctcaagtcc tgcaggcatg tacagcatgc
61 agctcgcatc ctgtgtcaca ttgacacttg tgctccttgt caacagcgca cccacttcaa
121 gctccacttc aagctctaca gcggaagcac agcagcagca gcagcagcag cagcagcagc
181 agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg atggagaatt
241 acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc aagcaggcca
301 cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg catgttctgg
361 atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc agcaatatca
421 gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa ttcgatgatg
481 agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa agcatcatct
541 caacaagccc tcaataacta tgtacctcct gcttacaaca cataaggctc tctatttatt
601 taaatattta actttaattt atttttggat gtattgttta ctatcttttg taactactag
661 tcttcagatg ataaatatgg atctttaaag attctttttg taagccccaa gggctcaaaa
721 atgttttaaa ctatttatct gaaattattt attatattga attgttaaat atcatgtgta
781 ggtagactca ttaataaaag tatttagatg attcaaatat aaataagctc agatgtctgt
841 catttttagg acagcacaaa gtaagcgcta aaataacttc tcagttattc ctgtgaactc
901 tatgttaatc agtgttttca agaaataaag ctctcctct
我现在用的引物：同源引物：g tgctccttgt caacagcgc 从89bp处开始
互补引物：是570--590的 gcttacaaca cataaggctc这一段的互补序列。
大家看看我的引物行吗？不行的话，帮忙设计一个，谢谢了！