帮忙设计一个引物，要求不高
丁香园论坛
991
设计的引物TM在60度左右，最重要是扩增片段要在340-355bp,谢了先
FEATURES Location/Qualifiers
source 1..939
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/chromosome="3"
/map="3 19.2 cM"
gene 1..939
/gene="Il2"
/note="synonym: Il-2"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
CDS 48..557
/gene="Il2"
/note="go_component: extracellular [goid 0005576]
[evidence IEA];
go_component: extracellular space [goid 0005615] [evidence
TAS] [pmid 12466851];
go_function: cytokine activity [goid 0005125] [evidence
IEA];
go_function: interleukin-2 receptor binding [goid 0005134]
[evidence TAS] [pmid 9741021];
go_function: growth factor activity [goid 0008083]
[evidence IEA];
go_function: hormone activity [goid 0005179] [evidence
TAS] [pmid 1830926];
go_process: defense response [goid 0006952] [evidence IMP]
[pmid 7589135];
go_process: inflammatory response [goid 0006954] [evidence
IMP] [pmid 9741021];
go_process: immune response [goid 0006955] [evidence TAS]
[pmid 8402910];
go_process: cellular defense response [goid 0006968]
[evidence TAS] [pmid 8402910];
go_process: cell proliferation [goid 0008283] [evidence
IMP] [pmid 8402910]"
/codon_start=1
/product="interleukin 2"
/protein_id="NP_032392.1"
/db_xref="GI:7110653"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
/translation="MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQ
QQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRH
VLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFC
QSIISTSPQ"
misc_feature 48..551
/gene="Il2"
/note="IL2; Region: Interleukin-2 family"
/db_xref="CDD:166"
sig_peptide 48..107
/gene="Il2"
/product="signal peptide (aa -21 to -1)"
mat_peptide 108..554
/gene="Il2"
/product="interleukin-2 (aa 1-133)"
repeat_region 150..185
/note="12 tandem repeats of CAG"
ORIGIN
1 atcacccttg ctaatcactc ctcacagtga cctcaagtcc tgcaggcatg tacagcatgc
61 agctcgcatc ctgtgtcaca ttgacacttg tgctccttgt caacagcgca cccacttcaa
121 gctccacttc aagctctaca gcggaagcac agcagcagca gcagcagcag cagcagcagc
181 agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg atggagaatt
241 acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc aagcaggcca
301 cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg catgttctgg
361 atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc agcaatatca
421 gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa ttcgatgatg
481 agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa agcatcatct
541 caacaagccc tcaataacta tgtacctcct gcttacaaca cataaggctc tctatttatt
601 taaatattta actttaattt atttttggat gtattgttta ctatcttttg taactactag
661 tcttcagatg ataaatatgg atctttaaag attctttttg taagccccaa gggctcaaaa
721 atgttttaaa ctatttatct gaaattattt attatattga attgttaaat atcatgtgta
781 ggtagactca ttaataaaag tatttagatg attcaaatat aaataagctc agatgtctgt
841 catttttagg acagcacaaa gtaagcgcta aaataacttc tcagttattc ctgtgaactc
901 tatgttaatc agtgttttca agaaataaag ctctcctct
FEATURES Location/Qualifiers
source 1..939
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/chromosome="3"
/map="3 19.2 cM"
gene 1..939
/gene="Il2"
/note="synonym: Il-2"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
CDS 48..557
/gene="Il2"
/note="go_component: extracellular [goid 0005576]
[evidence IEA];
go_component: extracellular space [goid 0005615] [evidence
TAS] [pmid 12466851];
go_function: cytokine activity [goid 0005125] [evidence
IEA];
go_function: interleukin-2 receptor binding [goid 0005134]
[evidence TAS] [pmid 9741021];
go_function: growth factor activity [goid 0008083]
[evidence IEA];
go_function: hormone activity [goid 0005179] [evidence
TAS] [pmid 1830926];
go_process: defense response [goid 0006952] [evidence IMP]
[pmid 7589135];
go_process: inflammatory response [goid 0006954] [evidence
IMP] [pmid 9741021];
go_process: immune response [goid 0006955] [evidence TAS]
[pmid 8402910];
go_process: cellular defense response [goid 0006968]
[evidence TAS] [pmid 8402910];
go_process: cell proliferation [goid 0008283] [evidence
IMP] [pmid 8402910]"
/codon_start=1
/product="interleukin 2"
/protein_id="NP_032392.1"
/db_xref="GI:7110653"
/db_xref="GeneID:16183"
/db_xref="LocusID:16183"
/db_xref="MGI:96548"
/translation="MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQ
QQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRH
VLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFC
QSIISTSPQ"
misc_feature 48..551
/gene="Il2"
/note="IL2; Region: Interleukin-2 family"
/db_xref="CDD:166"
sig_peptide 48..107
/gene="Il2"
/product="signal peptide (aa -21 to -1)"
mat_peptide 108..554
/gene="Il2"
/product="interleukin-2 (aa 1-133)"
repeat_region 150..185
/note="12 tandem repeats of CAG"
ORIGIN
1 atcacccttg ctaatcactc ctcacagtga cctcaagtcc tgcaggcatg tacagcatgc
61 agctcgcatc ctgtgtcaca ttgacacttg tgctccttgt caacagcgca cccacttcaa
121 gctccacttc aagctctaca gcggaagcac agcagcagca gcagcagcag cagcagcagc
181 agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg atggagaatt
241 acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc aagcaggcca
301 cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg catgttctgg
361 atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc agcaatatca
421 gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa ttcgatgatg
481 agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa agcatcatct
541 caacaagccc tcaataacta tgtacctcct gcttacaaca cataaggctc tctatttatt
601 taaatattta actttaattt atttttggat gtattgttta ctatcttttg taactactag
661 tcttcagatg ataaatatgg atctttaaag attctttttg taagccccaa gggctcaaaa
721 atgttttaaa ctatttatct gaaattattt attatattga attgttaaat atcatgtgta
781 ggtagactca ttaataaaag tatttagatg attcaaatat aaataagctc agatgtctgt
841 catttttagg acagcacaaa gtaagcgcta aaataacttc tcagttattc ctgtgaactc
901 tatgttaatc agtgttttca agaaataaag ctctcctct
