E-Z 96® M13 Isolation Vacuum Manifold Protocol
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实验试剂
1. Sterile deionized water (or TE buffer)
2. Absolute (96%-100%) ethanol
实验设备
1. Microcentrifuge capable of at least 10,000 x g
2. Sterile 15 mL centrifuge tubes
3. Sterile 1.7 mL centrifuge tubes
4. Water bath preheated at 60°C
实验步骤
1. Grow the M13 infected bacteria in a 2.2 mL 96-well culture plate (not supplied).
2. Spin down bacterial cells by centrifugation at 5000 rpm for 15 minutes at room temperature.
9. Next, apply the vacuum until all of the liquid passes through the membrane.