E-Z 96 X-press Plasmid Vacuum Protocol
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实验试剂
Materials to Be Provided by User
1. Nuclease Free 1.5 and 2.0 mL Centrifuge Tubes
实验设备
Materials to Be Provided by User
1. Tabletop with swinging-bucket rotor capable of 3,000 x g
2. Vacuum Manifold (Cat# Vac-03)
实验步骤
4. Add 500μl ICE-COLD XCL Lysis Buffer contains RNase A and lysozyme to each well of the plate.
Note: XCL Lysis Buffer must be ice cold to achieve maximum plasmid yield.
5. Completely resuspend the cell pellet by vortexing the plate at maximum speed for 30-60 seconds.
Note: It is critical to fully resuspend the cell pellet to obtain optimized DNA yield.
1) Place E-Z 96 Xpress Plate on the top plate of manifold;
2) Place a waste collection tray or 2 ml deep well plate inside the manifold base;
10. Add 500μl DLW Buffer (diluted with isopropanol) into Each well of E-Z 96 Xpress Plate.