E-Z 96 X-press Plasmid Spin Protocol
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实验试剂
Materials to Be Provided by User
1. Nuclease Free 1.5 and 2.0 mL Centrifuge Tubes
实验设备
Materials to Be Provided by User
1. Tabletop with swinging-bucket rotor capable of 3,000 x g
2. Vacuum Manifold (Cat# Vac-03)
实验步骤
1. Process the cell lysate by following step 1-6 from vacuum protocol.
4. Add 500μl DLW Buffer (diluted with isopropanol) into Each well of E-Z 96 Xpress Plate.
6. Place the E-Z 96 X-press Plate on a new a new 300μl 96-well collection plate (provided).
8. Centrifuge at 3000 x g for 5 minutes to elute plasmid DNA.
