E. Z. N.A.TM X-press Plasmid Protocol
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实验试剂
Materials to Be Provided by User
实验设备
Materials to Be Provided by User
1. Tabletop micro-centrifuge capable of 13,000 x g
2. Nuclease Free 1.5 and 2.0 mL Centrifuge Tubes
实验步骤
4. Add 500μl ICE-COLD XCL Lysis Buffer which contains RNase A and lysozyme.
Note: XCL Lysis Buffer must be ice cold to achieve maximum plasmid yield.
5. Completely resuspend the cell pellet by vortexing at maximum speed for 30-60 seconds.
Note: It is critical to fully resuspend the cell pellet to obtain optimized DNA yield.
7. Transfer the entire cell lysate into a X-press spin column inserted in a 2 ml collection tube.
8. Centrifuge at maximum speed (13,000 x g) for 1 minute.
9. Discard the flow-through liquid and re-use the collection tube.
10. Add 500μl DLW Buffer (diluted with isopropanol) into the Xpress spin column.
11. Centrifuge at maximum speed (13,000 x g) for 1 minute.
12. Discard the flow-through liquid and re-use the collection tube.
15. Centrifuge at maximum speed (13,000 x g ) for 1 minute to elute the plasmid DNA.
