E-Z 96® Viral RNA Protocol with Centrifugation
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实验原理
实验试剂
实验设备
2. RNase-free filter pipette tips
3. Reagent reservoirs for multichannel pipets
4. Centrifuge with suitable rotor for 96-well plate.
6. RNase-Free 1.2 ml 96-well plate
7. Adhesive sealing film for microplate
8. 2ml 96-well deep well plate
实验步骤
2. Pipet 10ul of reconstituted OB Protease solution into each well of the 96-well plate.
4. Seal the plate with sealing film. Incubate at room temperature for 5-10 minutes.
5. Spin at 500 x g briefly to collect any liquid.
9. Remove the sealing film and repeat steps 7 and 8.
15. Remove the sealing film. Repeat step 13 and 14 for second elution.