UNISTS的序列是否可以直接作为RT-PCR的引物？

我要做一个RT-PCR看看PEDF在组织中的表达

在uni-sts里找到PEDF的STS
请问是否可以直接拿

Forward primer: AGAACAAAGATGAACGGTCGGT
Reverse primer: ACCAACTCTTTGCAGGACATGA
来做RT-PCR的引物？
如何验证？
谢谢

http://www.ncbi.nlm.nih.gov/genome/sts/sts.cgi?uid=211288

UniSTS:211288 Links
RH127978

Primer Information

Forward primer: AGAACAAAGATGAACGGTCGGT
Reverse primer: ACCAACTCTTTGCAGGACATGA
PCR product size: 218 (bp), Rattus norvegicus

Rattus norvegicus
Name: RH127978
Also known as: AA859286


Cross References
Gene GeneID: 287526
Symbol: Serpinf1
Description: serine (or cysteine) peptidase inhibitor, clade F, member 1
Position: 10q24
UniGene Rn.16993 Serine (or cysteine) peptidase inhibitor, clade F, member 1
RHdb RH127978
RGD RGD:29867


Electronic PCR results
RefSeq mRNA (1)
NM_177927.2 1020 .. 1237

mRNA (3)
AY216659.1 1020 .. 1237
CN543106.1 192 .. 409
BC078686.1 1008 .. 1225

Genomic (1)
CM000081.1 62713616 .. 62714076

Working Draft phase 1 (from GenBank HTGS division) (2)
AC120096.6 124615 .. 125075
AC131871.3 243329 .. 243789

ESTs (5 of 18)[Show All Hits]
AA859286.1 191 .. 408
BG372839.1 143 .. 360
BG374703.1 192 .. 409
BI277814.1 192 .. 409
BI287673.1 192 .. 409

Whole Genome Shotgun sequences (1)
AABR03073187.1 110753 .. 111213