请各位站友帮我设计一下RT-PCR的引物

我要做RT-PCR，其基因的mRNA序列为:
CDS 1..7671
ORIGIN
1 atgccgccgc tcctggcgcc cctgctctgc ctggcgctgc tgcccgcgct cgccgcacga
61 ggcccgcgat gctcccagcc cggtgagacc tgcctgaatg gcgggaagtg tgaagcggcc
121 aatggcacgg aggcctgcgt ctgtggcggg gccttcgtgg gcccgcgatg ccaggacccc
181 aacccgtgcc tcagcacccc ctgcaagaac gccgggacat gccacgtggt ggaccgcaga
241 ggcgtggcag actatgcctg cagctgtgcc ctgggcttct ctgggcccct ctgcctgaca
301 cccctggaca atgcctgcct caccaacccc tgccgcaacg ggggcacctg cgacctgctc
361 acgctgacgg agtacaagtg ccgctgcccg cccggctggt cagggaaatc gtgccagcag
421 gctgacccgt gcgcctccaa cccctgcgcc aacggtggcc agtgcctgcc cttcgaggcc
481 tcctacatct gccactgccc acccagcttc catggcccca cctgccggca ggatgtcaac
541 gagtgtggcc agaagcccgg gctttgccgc cacggaggca cctgccacaa cgaggtcggc
601 tcctaccgct gcgtctgccg cgccacccac actggcccca actgcgagcg gccctacgtg
661 ccctgcagcc cctcgccctg ccagaacggg ggcacctgcc gccccacggg cgacgtcacc
721 cacgagtgtg cctgcctgcc aggcttcacc ggccagaact gtgaggaaaa tatcgacgat
781 tgtccaggaa acaactgcaa gaacgggggt gcctgtgtgg acggcgtgaa cacctacaac
841 tgccgctgcc cgccagagtg gacaggtcag tactgtaccg aggatgtgga cgagtgccag
901 ctgatgccaa atgcctgcca gaacggcggg acctgccaca acacccacgg tggctacaac
961 tgcgtgtgtg tcaacggctg gactggtgag gactgcagcg agaacattga tgactgtgcc
1021 agcgccgcct gcttccacgg cgccacctgc catgaccgtg tggcctcctt ctactgcgag
1081 tgtccccatg gccgcacagg tctgctgtgc cacctcaacg acgcatgcat cagcaacccc
1141 tgtaacgagg gctccaactg cgacaccaac cctgtcaatg gcaaggccat ctgcacctgc
1201 ccctcggggt acacgggccc ggcctgcagc caggacgtgg atgagtgctc gctgggtgcc
1261 aacccctgcg agcatgcggg caagtgcatc aacacgctgg gctccttcga gtgccagtgt
1321 ctgcagggct acacgggccc ccgatgcgag atcgacgtca acgagtgcgt ctcgaacccg
1381 tgccagaacg acgccacctg cctggaccag attggggagt tccagtgcat ctgcatgccc
1441 ggctacgagg gtgtgcactg cgaggtcaac acagacgagt gtgccagcag cccctgcctg
1501 cacaatggcc gctgcctgga caagatcaat gagttccagt gcgagtgccc cacgggcttc
1561 actgggcatc tgtgccagta cgatgtggac gagtgtgcca gcaccccctg caagaatggt
1621 gccaagtgcc tggacggacc caacacttac acctgtgtgt gcacggaagg gtacacgggg
1681 acgcactgcg aggtggacat cgatgagtgc gaccccgacc cctgccacta cggctcctgc
1741 aaggacggcg tcgccacctt cacctgcctc tgccgcccag gctacacggg ccaccactgc
1801 gagaccaaca tcaacgagtg ctccagccag ccctgccgcc acgggggcac ctgccaggac
1861 cgcgacaacg cctacctctg cttctgcctg aaggggacca caggacccaa ctgcgagatc
1921 aacctggatg actgtgccag cagcccctgc gactcgggca cctgtctgga caagatcgat
1981 ggctacgagt gtgcctgtga gccgggctac acagggagca tgtgtaacat caacatcgat
2041 gagtgtgcgg gcaacccctg ccacaacggg ggcacctgcg aggacggcat caatggcttc
2101 acctgccgct gccccgaggg ctaccacgac cccacctgcc tgtctgaggt caatgagtgc
2161 aacagcaacc cctgcgtcca cggggcctgc cgggacagcc tcaacgggta caagtgcgac
2221 tgtgaccctg ggtggagtgg gaccaactgt gacatcaaca acaatgagtg tgaatccaac
2281 ccttgtgtca acggcggcac ctgcaaagac atgaccagtg gctacgtgtg cacctgccgg
2341 gagggcttca gcggtcccaa ctgccagacc aacatcaacg agtgtgcgtc caacccatgt
2401 ctgaaccagg gcacgtgtat tgacgacgtt gccgggtaca agtgcaactg cctgctgccc
2461 tacacaggtg ccacgtgtga ggtggtgctg gccccgtgtg cccccagccc ctgcagaaac
2521 ggcggggagt gcaggcaatc cgaggactat gagagcttct cctgtgtctg ccccacgggc
2581 tggcaagcag ggcagacctg tgaggtcgac atcaacgagt gcgttctgag cccgtgccgg
2641 cacggcgcat cctgccagaa cacccacggc ggctaccgct gccactgcca ggccggctac
2701 agtgggcgca actgcgagac cgacatcgac gactgccggc ccaacccgtg tcacaacggg
2761 ggctcctgca cagacggcat caacacggcc ttctgcgact gcctgcccgg cttccggggc
2821 actttctgtg aggaggacat caacgagtgt gccagtgacc cctgccgcaa cggggccaac
2881 tgcacggact gcgtggacag ctacacgtgc acctgccccg caggcttcag cgggatccac
2941 tgtgagaaca acacgcctga ctgcacagag agctcctgct tcaacggtgg cacctgcgtg
3001 gacggcatca actcgttcac ctgcctgtgt ccacccggct tcacgggcag ctactgccag
3061 cacgatgtca atgagtgcga ctcacagccc tgcctgcatg gcggcacctg tcaggacggc
3121 tgcggctcct acaggtgcac ctgcccccag ggctacactg gccccaactg ccagaacctt
3181 gtgcactggt gtgactcctc gccctgcaag aacggcggca aatgctggca gacccacacc
3241 cagtaccgct gcgagtgccc cagcggctgg accggccttt actgcgacgt gcccagcgtg
3301 tcctgtgagg tggctgcgca gcgacaaggt gttgacgttg cccgcctgtg ccagcatgga
3361 gggctctgtg tggacgcggg caacacgcac cactgccgct gccaggcggg ctacacaggc
3421 agctactgtg aggacctggt ggacgagtgc tcacccagcc cctgccagaa cggggccacc
3481 tgcacggact acctgggcgg ctactcctgc aagtgcgtgg ccggctacca cggggtgaac
3541 tgctctgagg agatcgacga gtgcctctcc cacccctgcc agaacggggg cacctgcctc
3601 gacctcccca acacctacaa gtgctcctgc ccacggggca ctcagggtgt gcactgtgag
3661 atcaacgtgg acgactgcaa tccccccgtt gaccccgtgt cccggagccc caagtgcttt
3721 aacaacggca cctgcgtgga ccaggtgggc ggctacagct gcacctgccc gccgggcttc
3781 gtgggtgagc gctgtgaggg ggatgtcaac gagtgcctgt ccaatccctg cgacgcccgt
3841 ggcacccaga actgcgtgca gcgcgtcaat gacttccact gcgagtgccg tgctggtcac
3901 accgggcgcc gctgcgagtc cgtcatcaat ggctgcaaag gcaagccctg caagaatggg
3961 ggcacctgcg ccgtggcctc caacaccgcc cgcgggttca tctgcaagtg ccctgcgggc
4021 ttcgagggcg ccacgtgtga gaatgacgct cgtacctgcg gcagcctgcg ctgcctcaac
4081 ggcggcacat gcatctccgg cccgcgcagc cccacctgcc tgtgcctggg ccccttcacg
4141 ggccccgaat gccagttccc ggccagcagc ccctgcctgg gcggcaaccc ctgctacaac
4201 caggggacct gtgagcccac atccgagagc cccttctacc gttgcctgtg ccccgccaaa
4261 ttcaacgggc tcttgtgcca catcctggac tacagcttcg ggggtggggc cgggcgcgac
4321 atccccccgc cgctgatcga ggaggcgtgc gagctgcccg agtgccagga ggacgcgggc
4381 aacaaggtct gcagcctgca gtgcaacaac cacgcgtgcg gctgggacgg cggtgactgc
4441 tccctcaact tcaatgaccc ctggaagaac tgcacgcagt ctctgcagtg ctggaagtac
4501 ttcagtgacg gccactgtga cagccagtgc aactcagccg gctgcctctt cgacggcttt
4561 gactgccagc gtgcggaagg ccagtgcaac cccctgtacg accagtactg caaggaccac
4621 ttcagcgacg ggcactgcga ccagggctgc aacagcgcgg agtgcgagtg ggacgggctg
4681 gactgtgcgg agcatgtacc cgagaggctg gcggccggca cgctggtggt ggtggtgctg
4741 atgccgccgg agcagctgcg caacagctcc ttccacttcc tgcgggagct cagccgcgtg
4801 ctgcacacca acgtggtctt caagcgtgac gcacacggcc agcagatgat cttcccctac
4861 tacggccgcg aggaggagct gcgcaagcac cccatcaagc gtgccgccga gggctgggcc
4921 gcacctgacg ccctgctggg ccaggtgaag gcctcgctgc tccctggtgg cagcgagggt
4981 gggcggcggc ggagggagct ggaccccatg gacgtccgcg gctccatcgt ctacctggag
5041 attgacaacc ggcagtgtgt gcaggcctcc tcgcagtgct tccagagtgc caccgacgtg
5101 gccgcattcc tgggagcgct cgcctcgctg ggcagcctca acatccccta caagatcgag
5161 gccgtgcaga gtgagaccgt ggagccgccc ccgccggcgc agctgcactt catgtacgtg
5221 gcggcggccg cctttgtgct tctgttcttc gtgggctgcg gggtgctgct gtcccgcaag
5281 cgccggcggc agcatggcca gctctggttc cctgagggct tcaaagtgtc tgaggccagc
5341 aagaagaagc ggcgggagcc cctcggcgag gactccgtgg gcctcaagcc cctgaagaac
5401 gcttcagacg gtgccctcat ggacgacaac cagaatgagt ggggggacga ggacctggag
5461 accaagaagt tccggttcga ggagcccgtg gttctgcctg acctggacga ccagacagac
5521 caccggcagt ggactcagca gcacctggat gccgctgacc tgcgcatgtc tgccatggcc
5581 cccacaccgc cccagggtga ggttgacgcc gactgcatgg acgtcaatgt ccgcgggcct
5641 gatggcttca ccccgctcat gatcgcctcc tgcagcgggg gcggcctgga gacgggcaac
5701 agcgaggaag aggaggacgc gccggccgtc atctccgact tcatctacca gggcgccagc
5761 ctgcacaacc agacagaccg cacgggcgag accgccttgc acctggccgc ccgctactca
5821 cgctctgatg ccgccaagcg cctgctggag gccagcgcag atgccaacat ccaggacaac
5881 atgggccgca ccccgctgca tgcggctgtg tctgccgacg cacaaggtgt cttccagatc
5941 ctgatccgga accgagccac agacctggat gcccgcatgc atgatggcac gacgccactg
6001 atcctggctg cccgcctggc cgtggagggc atgctggagg acctcatcaa ctcacacgcc
6061 gacgtcaacg ccgtagatga cctgggcaag tccgccctgc actgggccgc cgccgtgaac
6121 aatgtggatg ccgcagttgt gctcctgaag aacggggcta acaaagatat gcagaacaac
6181 agggaggaga cacccctgtt tctggccgcc cgggagggca gctacgagac cgccaaggtg
6241 ctgctggacc actttgccaa ccgggacatc acggatcata tggaccgcct gccgcgcgac
6301 atcgcacagg agcgcatgca tcacgacatc gtgaggctgc tggacgagta caacctggtg
6361 cgcagcccgc agctgcacgg agccccgctg gggggcacgc ccaccctgtc gcccccgctc
6421 tgctcgccca acggctacct gggcagcctc aagcccggcg tgcagggcaa gaaggtccgc
6481 aagcccagca gcaaaggcct ggcctgtgga agcaaggagg ccaaggacct caaggcacgg
6541 aggaagaagt cccaggacgg caagggctgc ctgctggaca gctccggcat gctctcgccc
6601 gtggactccc tggagtcacc ccatggctac ctgtcagacg tggcctcgcc gccactgctg
6661 ccctccccgt tccagcagtc tccgtccgtg cccctcaacc acctgcctgg gatgcccgac
6721 acccacctgg gcatcgggca cctgaacgtg gcggccaagc ccgagatggc ggcgctgggt
6781 gggggcggcc ggctggcctt tgagactggc ccacctcgtc tctcccacct gcctgtggcc
6841 tctggcacca gcaccgtcct gggctccagc agcggagggg ccctgaattt cactgtgggc
6901 gggtccacca gtttgaatgg tcaatgcgag tggctgtccc ggctgcagag cggcatggtg
6961 ccgaaccaat acaaccctct gcgggggagt gtggcaccag gccccctgag cacacaggcc
7021 ccctccctgc agcatggcat ggtaggcccg ctgcacagta gccttgctgc cagcgccctg
7081 tcccagatga tgagctacca gggcctgccc agcacccggc tggccaccca gcctcacctg
7141 gtgcagaccc agcaggtgca gccacaaaac ttacagatgc agcagcagaa cctgcagcca
7201 gcaaacatcc agcagcagca aagcctgcag ccgccaccac caccaccaca gccgcacctt
7261 ggcgtgagct cagcagccag cggccacctg ggccggagct tcctgagtgg agagccgagc
7321 caggcagacg tgcagccact gggccccagc agcctggcgg tgcacactat tctgccccag
7381 gagagccccg ccctgcccac gtcgctgcca tcctcgctgg tcccacccgt gaccgcagcc
7441 cagttcctga cgcccccctc gcagcacagc tactcctcgc ctgtggacaa cacccccagc
7501 caccagctac aggtgcctga gcaccccttc ctcaccccgt cccctgagtc ccctgaccag
7561 tggtccagct cgtccccgca ttccaacgtc tccgactggt ccgagggcgt ctccagccct
7621 cccaccagca tgcagtccca gatcgcccgc attccggagg ccttcaagta aacggcgcgc
7681 cccacgagac cccggcttcc tttcccaagc cttcgggcgt ctgtgtgcgc tctgtggatg
7741 ccagggccga ccagaggagc ctttttaaaa cacatgtttt tatacaaaat aagaacgagg
7801 attttaattt tttttagtat ttatttatgt acttttattt tacacagaaa cactgccttt
7861 ttatttatat gtactgtttt atctggcccc aggtagaaac ttttatctat tctgagaaaa
7921 caagcaagtt ctgagagcca gggttttcct acgtaggatg aaaagattct tctgtgttta
7981 taaaatataa acaaagattc atgatttata aatgccattt atttattgat tccttttttc
8041 aaaatccaaa aagaaatgat gttggagaag ggaagttgaa cgagcatagt ccaaaaagct
8101 cctggggcgt ccaggccgcg ccctttcccc gacgcccacc caaccccaag ccagcccggc
8161 cgctccacca gcatcacctg cctgttagga gaagctgcat ccagaggcaa acggaggcaa
8221 agctggctca ccttccgcac gcggattaat ttgcatctga aataggaaac aagtgaaagc
8281 atatgggtta gatgttgcca tgtgttttag atggtttctt gcaagcatgc ttgtgaaaat
8341 gtgttctcgg agtgtgtatg ccaagagtgc acccatggta ccaatcatga atctttgttt
8401 caggttcagt attatgtagt tgttcgttgg ttatacaagt tcttggtccc tccagaacca
8461 ccccggcccc ctgcccgttc ttgaaatgta ggcatcatgc atgtcaaaca tgagatgtgt
8521 ggactgtggc acttgcctgg gtcacacacg gaggcatcct acccttttct ggggaaagac
8581 actgcctggg ctgaccccgg tggcggcccc agcacctcag cctgcacagt gtcccccagg
8641 ttccgaagaa gatgctccag caacacagcc tgggccccag ctcgcgggac ccgacccccc
8701 gtgggctccc gtgttttgta ggagacttgc cagagccggg cacattgagc tgtgcaacgc
8761 cgtgggctgc gtcctttggt cctgtccccg cagccctggc agggggcatg cggtcgggca
8821 ggggctggag ggaggcgggg gctgcccttg ggccacccct cctagtttgg gaggagcaga
8881 tttttgcaat accaagtata gcctatggca gaaaaaatgt ctgtaaatat gtttttaaag
8941 gtggattttg tttaaaaaat cttaatgaat gagtctgttg tgtgtcatgc cagtgaggga
9001 cgtcagactt ggctcagctc ggggagcctt agccgcccat gcactgggga cgctccgctg
9061 ccgtgccgcc tgcactcctc agggcagcct cccccggctc tacgggggcc gcgtggtgcc
9121 atccccaggg ggcatgacca gatgcgtccc aagatgttga tttttactgt gttttataaa
9181 atagagtgta gtttacagaa aaagacttta aaagtgatct acatgaggaa ctgtagatga
9241 tgtatttttt tcatcttttt tgttaactga tttgcaataa aaatgatact gatggtgaaa
9301 aaaaaaaaaa aa
谢谢！