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请帮看一下blast结果

丁香园论坛

2191
这是我设计的P53基因引物blast结果,请教高手这引物可用吗?
Distribution of 104 Blast Hits on the Query Sequence


--------------------------------------------------------------------------------

Score E
Sequences producing significant alignments: (bits) Value

gi|51475426|ref|XM_496493.1| PREDICTED: Homo sapiens simila... 38 0.69
gi|34740425|gb|AC122299.5| Mus musculus BAC clone RP23-270L... 36 2.7
gi|38229493|gb|AC133210.3| Mus musculus chromosome UNK clon... 36 2.7
gi|38142554|gb|AC114987.10| Mus musculus chromosome 3, clon... 36 2.7
gi|50727082|gb|AC132240.3| Mus musculus chromosome 3 clone ... 36 2.7
gi|52694628|emb|CR382328.14| Mouse DNA sequence from clone ... 36 2.7
gi|45680637|gb|AC108428.13| Mus musculus chromosome 3, clon... 36 2.7
gi|20330185|emb|AL607074.13| Mouse DNA sequence from clone ... 36 2.7
gi|24395202|emb|AL844856.6| Mouse DNA sequence from clone R... 36 2.7
gi|9690342|gb|AC058790.14| Homo sapiens chromosome 22 clone... 34 11
gi|7940355|gb|AC007663.29| Homo sapiens chromosome 22 clone... 34 11
gi|14209695|gb|AC090431.9| Mus Musculus Strain C57BL6/J chr... 34 11
gi|28376744|gb|AC090966.21| Papio anubis clone rp41-5c11, c... 34 11
gi|18698849|gb|AC021518.12| Homo sapiens chromosome 8, clon... 34 11
gi|25704382|gb|AC092532.17| Papio anubis clone rp41-110d13,... 34 11
gi|46849819|gb|AC111046.28| Mus musculus chromosome 10, clo... 34 11
gi|28193395|emb|AL627238.15| Mouse DNA sequence from clone ... 34 11
gi|13899427|gb|AC013492.5|AC013492 Homo sapiens chromosome ... 34 11
gi|22552841|emb|AL713894.12| Mouse DNA sequence from clone ... 34 11
gi|1694657|dbj|D89004.1| Glirulus japonicus mitochondrial D... 34 11
gi|1694653|dbj|D89002.1| Glirulus japonicus mitochondrial D... 34 11
gi|1694651|dbj|D89001.1| Glirulus japonicus mitochondrial D... 34 11
gi|1694649|dbj|D89000.1| Glirulus japonicus mitochondrial D... 34 11
gi|13359178|dbj|AB051440.1| Homo sapiens mRNA for KIAA1653 ... 34 11
gi|33354424|gb|AC124171.3| Mus musculus BAC clone RP23-167E... 32 42
gi|22475928|gb|AC131723.1| Mus musculus BAC clone RP23-144E... 32 42
gi|56382145|gb|AC116386.9| Mus musculus chromosome 15, clon... 32 42
gi|56044726|gb|AC102164.7| Mus musculus chromosome 3, clone... 32 42
gi|19033960|gb|AC073968.8| Homo sapiens BAC clone RP11-111G... 32 42
gi|19033989|gb|AC073325.9| Homo sapiens BAC clone RP11-351I... 32 42
gi|34536782|gb|AC113275.8| Mus musculus chromosome 8, clone... 32 42
gi|34556282|gb|AC115754.7| Mus musculus chromosome 12, clon... 32 42
gi|55700260|gb|AC127582.4| Mus musculus chromosome 12 clone... 32 42
gi|31416115|gb|AC145090.1| Pan troglodytes BAC clone RP43-2... 32 42
gi|49066500|gb|AY452871.1| Fusarium meridionale strain NRRL... 32 42
gi|32404981|ref|XM_323103.1| Neurospora crassa strain OR74A 32 42
gi|52078011|gb|AC132465.3| Mus musculus chromosome 10 clone... 32 42
gi|45592966|gb|AC132485.2| Oryza sativa (japonica cultivar-... 32 42
gi|24137491|gb|AC008103.29| Homo sapiens chromosome 22 clon... 32 42
gi|7229748|gb|AC007326.28| Homo sapiens chromosome 22 clone... 32 42
gi|7229741|gb|AC007325.51| Homo sapiens chromosome 22 clone... 32 42
gi|50927702|gb|BC079100.1| Rattus norvegicus cDNA clone MGC... 32 42
gi|7022569|dbj|AK001359.1| Homo sapiens cDNA FLJ10497 fis, ... 32 42
gi|18121489|emb|AL513022.19| Mouse DNA sequence from clone ... 32 42
gi|20260774|gb|AC025198.12| Homo sapiens chromosome 17, clo... 32 42
gi|19849359|gb|AC091549.11| Homo sapiens chromosome 8, clon... 32 42
gi|2814534|emb|Z83216.1|CEC08F11 Caenorhabditis elegans cos... 32 42
gi|11595616|emb|AL451018.1|NC99H12 Neurospora crassa DNA li... 32 42
gi|12718469|emb|AL513466.1|NCB18D24 Neurospora crassa DNA l... 32 42
gi|13346588|gb|AC020601.10| Homo sapiens BAC clone RP11-451... 32 42
gi|31616680|emb|AL837516.14| Mouse DNA sequence from clone ... 32 42
gi|34365799|gb|AC130603.2| Oryza sativa (japonica cultivar-... 32 42
gi|28446431|emb|AL935336.11| Mouse DNA sequence from clone ... 32 42
gi|12643020|gb|AC080018.14|AC080018 Mus musculus 3 BAC RP23... 32 42
gi|25251466|emb|AL731650.26| Mouse DNA sequence from clone ... 32 42
gi|46805765|dbj|AP004853.3| Oryza sativa (japonica cultivar... 32 42
gi|340702|gb|M21929.1|PB2REPZZ Plasmid pUB112 (from Staphyl... 32 42
gi|41015439|gb|AC105947.11| Mus musculus chromosome 18, clo... 32 42
gi|1694655|dbj|D89003.1| Glirulus japonicus mitochondrial D... 32 42
gi|38488893|gb|AC131667.4| Mus musculus BAC clone RP23-290D... 32 42
gi|19571952|emb|AL606832.4| Mouse DNA sequence from clone R... 32 42
gi|56566254|gb|AF542529.2| Cryptococcus neoformans var. gru... 30 167
gi|34495030|gb|AC126933.3| Mus musculus BAC clone RP24-117M... 30 167
gi|38502332|gb|AC092327.4| Homo sapiens chromosome 16 clone... 30 167
gi|17569176|ref|NM_076290.1| Caenorhabditis elegans mo25 pr... 30 167
gi|32565346|ref|NM_065694.2| Caenorhabditis elegans proteas... 30 167
gi|27877431|gb|AC122307.4| Mus musculus BAC clone RP23-287C... 30 167
gi|22296771|gb|AC121576.2| Mus musculus BAC clone RP23-244D... 30 167
gi|34874138|ref|XM_224181.2| Rattus norvegicus similar to C... 30 167
gi|37859177|gb|AC113123.8| Mus musculus chromosome 8, clone... 30 167
gi|55831575|gb|AC140673.4| Mus musculus chromosome 15 clone... 30 167
gi|28851051|gb|AE016858.1| Pseudomonas syringae pv. tomato ... 30 167
gi|31127182|gb|BC052790.1| Mus musculus RIKEN cDNA 1500001L... 30 167
gi|20381486|gb|BC027639.1| Mus musculus RIKEN cDNA 2310004N... 30 167
gi|28381649|gb|AE003509.4| Drosophila melanogaster chromoso... 30 167
gi|37059972|gb|AC113287.8| Mus musculus chromosome 3, clone... 30 167
gi|27358029|gb|AE016808.1| Vibrio vulnificus CMCP6 chromoso... 30 167
gi|34787564|gb|AC146245.2| Pan troglodytes BAC clone RP43-3... 30 167
gi|31209498|ref|XM_313716.1| Anopheles gambiae ENSANGP00000... 30 167
gi|50900275|ref|XM_450926.1| Oryza sativa (japonica cultiva... 30 167
gi|54792871|gb|AC133943.3| Mus musculus chromosome 3 clone ... 30 167
gi|31563312|gb|AC095504.7| Rattus norvegicus 2 BAC CH230-7P... 30 167
gi|46425363|emb|AJ586576.1| Xanthomonas albilineans gene cl... 30 167
gi|29897592|gb|AE017011.1| Bacillus cereus ATCC 14579 secti... 30 167
gi|53749573|gb|AC103637.11| Mus musculus chromosome 3, clon... 30 167
gi|46518553|gb|AC111016.2| Oryza sativa (japonica cultivar-... 30 167
gi|42459789|emb|BX833655.1|CNS0A20G Arabidopsis thaliana Fu... 30 167
gi|42457775|emb|BX833770.1|CNS0A0K1 Arabidopsis thaliana Fu... 30 167
gi|51768437|ref|XM_127738.4| PREDICTED: Mus musculus RIKEN ... 30 167
gi|51766539|ref|XM_181318.5| PREDICTED: Mus musculus RIKEN ... 30 167
gi|23379131|gb|AC132810.2| Homo sapiens chromosome 3 clone ... 30 167
gi|51471746|ref|XM_033371.5| PREDICTED: Homo sapiens chromo... 30 167
gi|29788912|gb|AC115284.4| Homo sapiens chromosome 3 clone ... 30 167
gi|49258291|gb|AC129775.4| Mus musculus BAC clone RP23-174N... 30 167
gi|34221810|emb|AL954130.8| Zebrafish DNA sequence from clo... 30 167
gi|48374345|gb|AY590104.1| Enterobacter cloacae Anr-F and A... 30 167
gi|18057076|gb|AC024591.4| Homo sapiens chromosome 16 clone... 30 167
gi|53130795|emb|AJ720068.1| Gallus gallus mRNA for hypothet... 30 167
gi|20160968|dbj|AP003300.4| Oryza sativa (japonica cultivar... 30 167

Alignments


>gi|51475426|ref|XM_496493.1| PREDICTED: Homo sapiens similar to proline dehydrogenase (oxidase)
1; tumor protein p53 inducible protein 6; p53 induced
protein; proline oxidase 2; proline dehydrogenase
(proline oxidase ) (LOC440792), mRNA
Length = 1140

Score = 38.2 bits (19), Expect = 0.69
Identities = 19/19 (100%)
Strand = Plus / Minus


Query: 18 gcccacccaatcgtcaaac 36
|||||||||||||||||||
Sbjct: 264 gcccacccaatcgtcaaac 246

>gi|34740425|gb|AC122299.5| Mus musculus BAC clone RP23-270L23 from chromosome 5, complete sequence
Length = 209091

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Minus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 105092 aggaaagtctcaaagcccaccc 105071

>gi|38229493|gb|AC133210.3| Mus musculus chromosome UNK clone RP23-27L12, complete sequence
Length = 210702

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Minus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 79430 aggaaagtctcaaagcccaccc 79409

>gi|38142554|gb|AC114987.10| Mus musculus chromosome 3, clone RP23-81D7, complete sequence
Length = 179717

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 159802 aggaaagtctcaaagcccaccc 159823

>gi|50727082|gb|AC132240.3| Mus musculus chromosome 3 clone RP24-411A22, complete sequence
Length = 182978

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 167707 aggaaagtctcaaagcccaccc 167728

>gi|52694628|emb|CR382328.14| Mouse DNA sequence from clone RP23-315J18 on chromosome X, complete
sequence
Length = 215381

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 47363 aggaaagtctcaaagcccaccc 47384

>gi|45680637|gb|AC108428.13| Mus musculus chromosome 3, clone RP23-145M5, complete sequence
Length = 200331

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 43051 aggaaagtctcaaagcccaccc 43072

>gi|20330185|emb|AL607074.13| Mouse DNA sequence from clone RP23-174M9 on chromosome 4, complete
sequence
Length = 204428

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Minus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 164300 aggaaagtctcaaagcccaccc 164279

>gi|24395202|emb|AL844856.6| Mouse DNA sequence from clone RP23-377G1 on chromosome 4, complete
sequence
Length = 183318

Score = 36.2 bits (18), Expect = 2.7
Identities = 21/22 (95%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagcccaccc 25
||||||||| ||||||||||||
Sbjct: 39316 aggaaagtctcaaagcccaccc 39337

>gi|9690342|gb|AC058790.14| Homo sapiens chromosome 22 clone b518b9 map 22q11, complete sequence
Length = 184929

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Minus


Query: 20 ccacccaatcgtcaaac 36
|||||||||||||||||
Sbjct: 164307 ccacccaatcgtcaaac 164291

>gi|7940355|gb|AC007663.29| Homo sapiens chromosome 22 clone b444p24 map 22q11, complete sequence
Length = 168239

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Minus


Query: 20 ccacccaatcgtcaaac 36
|||||||||||||||||
Sbjct: 152417 ccacccaatcgtcaaac 152401

>gi|14209695|gb|AC090431.9| Mus Musculus Strain C57BL6/J chromosome 14 RP23-324C9, complete sequence
Length = 227949

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Minus


Query: 1 tcaaggaaagtcgcaaa 17
|||||||||||||||||
Sbjct: 157498 tcaaggaaagtcgcaaa 157482

>gi|28376744|gb|AC090966.21| Papio anubis clone rp41-5c11, complete sequence
Length = 202399

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 20 ccacccaatcgtcaaac 36
|||||||||||||||||
Sbjct: 114910 ccacccaatcgtcaaac 114926

>gi|18698849|gb|AC021518.12| Homo sapiens chromosome 8, clone RP11-313D2, complete sequence
Length = 169566

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 13 gcaaagcccacccaatc 29
|||||||||||||||||
Sbjct: 161470 gcaaagcccacccaatc 161486

>gi|25704382|gb|AC092532.17| Papio anubis clone rp41-110d13, complete sequence
Length = 157944

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 20 ccacccaatcgtcaaac 36
|||||||||||||||||
Sbjct: 81655 ccacccaatcgtcaaac 81671

>gi|46849819|gb|AC111046.28| Mus musculus chromosome 10, clone RP23-479A12, complete sequence
Length = 231134

Score = 34.2 bits (17), Expect = 11
Identities = 20/21 (95%)
Strand = Plus / Plus


Query: 5 ggaaagtcgcaaagcccaccc 25
|||||||| ||||||||||||
Sbjct: 173209 ggaaagtctcaaagcccaccc 173229

>gi|28193395|emb|AL627238.15| Mouse DNA sequence from clone RP23-46M23 on chromosome 4, complete
sequence
Length = 210380

Score = 34.2 bits (17), Expect = 11
Identities = 20/21 (95%)
Strand = Plus / Plus


Query: 6 gaaagtcgcaaagcccaccca 26
||||||| |||||||||||||
Sbjct: 39278 gaaagtctcaaagcccaccca 39298

>gi|13899427|gb|AC013492.5|AC013492 Homo sapiens chromosome , clone RP11-32K4, complete sequence
Length = 167174

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 13 gcaaagcccacccaatc 29
|||||||||||||||||
Sbjct: 92837 gcaaagcccacccaatc 92853

>gi|22552841|emb|AL713894.12| Mouse DNA sequence from clone RP23-48J18 on chromosome X, complete
sequence
Length = 264395

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 13 gcaaagcccacccaatc 29
|||||||||||||||||
Sbjct: 182050 gcaaagcccacccaatc 182066

>gi|1694657|dbj|D89004.1| Glirulus japonicus mitochondrial DNA for cytochrome b, partial
cds
Length = 402

Score = 34.2 bits (17), Expect = 11
Identities = 23/25 (92%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccacccaatcgtcaaa 35
||||||| |||||||||||| ||||
Sbjct: 12 tcgcaaaacccacccaatcgccaaa 36

>gi|1694653|dbj|D89002.1| Glirulus japonicus mitochondrial DNA for cytochrome b, partial
cds
Length = 402

Score = 34.2 bits (17), Expect = 11
Identities = 23/25 (92%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccacccaatcgtcaaa 35
||||||| ||||||||||| |||||
Sbjct: 12 tcgcaaaacccacccaatcatcaaa 36

>gi|1694651|dbj|D89001.1| Glirulus japonicus mitochondrial DNA for cytochrome b, partial
cds
Length = 402

Score = 34.2 bits (17), Expect = 11
Identities = 23/25 (92%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccacccaatcgtcaaa 35
||||||| ||||||||||| |||||
Sbjct: 12 tcgcaaaacccacccaatcatcaaa 36

>gi|1694649|dbj|D89000.1| Glirulus japonicus mitochondrial DNA for cytochrome b, partial
cds
Length = 402

Score = 34.2 bits (17), Expect = 11
Identities = 23/25 (92%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccacccaatcgtcaaa 35
||||||| |||||||||||| ||||
Sbjct: 12 tcgcaaaacccacccaatcgccaaa 36

>gi|13359178|dbj|AB051440.1| Homo sapiens mRNA for KIAA1653 protein, partial cds
Length = 5868

Score = 34.2 bits (17), Expect = 11
Identities = 17/17 (100%)
Strand = Plus / Minus


Query: 20 ccacccaatcgtcaaac 36
|||||||||||||||||
Sbjct: 2065 ccacccaatcgtcaaac 2049

>gi|33354424|gb|AC124171.3| Mus musculus BAC clone RP23-167E23 from chromosome 13, complete sequence
Length = 198242

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 2 caaggaaagtcgcaaa 17
||||||||||||||||
Sbjct: 129496 caaggaaagtcgcaaa 129481

>gi|22475928|gb|AC131723.1| Mus musculus BAC clone RP23-144E24 from 1, complete sequence
Length = 200656

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 4 aggaaagtcgcaaagc 19
||||||||||||||||
Sbjct: 11670 aggaaagtcgcaaagc 11685

>gi|56382145|gb|AC116386.9| Mus musculus chromosome 15, clone RP23-37B16, complete sequence
Length = 185556

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccaccca 26
||||||||||||||||
Sbjct: 24721 tcgcaaagcccaccca 24736

>gi|56044726|gb|AC102164.7| Mus musculus chromosome 3, clone RP23-438M14, complete sequence
Length = 184952

Score = 32.2 bits (16), Expect = 42
Identities = 19/20 (95%)
Strand = Plus / Minus


Query: 6 gaaagtcgcaaagcccaccc 25
||||||| ||||||||||||
Sbjct: 144213 gaaagtctcaaagcccaccc 144194

>gi|19033960|gb|AC073968.8| Homo sapiens BAC clone RP11-111G20 from 7, complete sequence
Length = 92245

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 13 gcaaagcccacccaat 28
||||||||||||||||
Sbjct: 189 gcaaagcccacccaat 174

>gi|19033989|gb|AC073325.9| Homo sapiens BAC clone RP11-351I12 from 7, complete sequence
Length = 168872

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 13 gcaaagcccacccaat 28
||||||||||||||||
Sbjct: 168861 gcaaagcccacccaat 168846

>gi|34536782|gb|AC113275.8| Mus musculus chromosome 8, clone RP23-383K23, complete sequence
Length = 253984

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 14 caaagcccacccaatc 29
||||||||||||||||
Sbjct: 36897 caaagcccacccaatc 36882

>gi|34556282|gb|AC115754.7| Mus musculus chromosome 12, clone RP23-34D20, complete sequence
Length = 217173

Score = 32.2 bits (16), Expect = 42
Identities = 19/20 (95%)
Strand = Plus / Minus


Query: 4 aggaaagtcgcaaagcccac 23
||||||||| ||||||||||
Sbjct: 161969 aggaaagtctcaaagcccac 161950

>gi|55700260|gb|AC127582.4| Mus musculus chromosome 12 clone RP24-89J3, complete sequence
Length = 217949

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 13 gcaaagcccacccaat 28
||||||||||||||||
Sbjct: 66676 gcaaagcccacccaat 66691

Score = 28.2 bits (14), Expect = 662
Identities = 14/14 (100%)
Strand = Plus / Plus


Query: 16 aagcccacccaatc 29
||||||||||||||
Sbjct: 69238 aagcccacccaatc 69251

>gi|31416115|gb|AC145090.1| Pan troglodytes BAC clone RP43-2G11 from 7, complete sequence
Length = 173759

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 13 gcaaagcccacccaat 28
||||||||||||||||
Sbjct: 108348 gcaaagcccacccaat 108333

>gi|49066500|gb|AY452871.1| Fusarium meridionale strain NRRL 28436 mating type protein MAT1-1-3,
mating type protein MAT1-1-2, mating type protein
MAT1-1-1, and mating type protein MAT1-2-1 genes, partial
sequence
Length = 5908

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccaccca 26
||||||||||||||||
Sbjct: 3238 tcgcaaagcccaccca 3253

>gi|32404981|ref|XM_323103.1| Neurospora crassa strain OR74A
Length = 879

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 11 tcgcaaagcccaccca 26
||||||||||||||||
Sbjct: 667 tcgcaaagcccaccca 652

>gi|52078011|gb|AC132465.3| Mus musculus chromosome 10 clone RP23-58D1, complete sequence
Length = 218313

Score = 32.2 bits (16), Expect = 42
Identities = 19/20 (95%)
Strand = Plus / Plus


Query: 3 aaggaaagtcgcaaagccca 22
|||||||||| |||||||||
Sbjct: 119500 aaggaaagtctcaaagccca 119519

>gi|45592966|gb|AC132485.2| Oryza sativa (japonica cultivar-group) chromosome 5 clone P0022D06,
complete sequence
Length = 142068

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 21 cacccaatcgtcaaac 36
||||||||||||||||
Sbjct: 114697 cacccaatcgtcaaac 114712

>gi|24137491|gb|AC008103.29| Homo sapiens chromosome 22 clone pac699j1 map 22q12, complete sequence
Length = 98557

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 21 cacccaatcgtcaaac 36
||||||||||||||||
Sbjct: 74554 cacccaatcgtcaaac 74569

>gi|7229748|gb|AC007326.28| Homo sapiens chromosome 22 clone p423 map 22q11, complete sequence
Length = 102898

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 21 cacccaatcgtcaaac 36
||||||||||||||||
Sbjct: 18361 cacccaatcgtcaaac 18376

>gi|7229741|gb|AC007325.51| Homo sapiens chromosome 22 clone b48 map 22q11, complete sequence
Length = 165050

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 21 cacccaatcgtcaaac 36
||||||||||||||||
Sbjct: 145458 cacccaatcgtcaaac 145473

>gi|50927702|gb|BC079100.1| Rattus norvegicus cDNA clone MGC:94080 IMAGE:7124466, complete cds
Length = 2461

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 14 caaagcccacccaatc 29
||||||||||||||||
Sbjct: 2250 caaagcccacccaatc 2235

>gi|7022569|dbj|AK001359.1| Homo sapiens cDNA FLJ10497 fis, clone NT2RP2000310, highly similar to
Human proline dehydrogenase/proline oxidase mRNA
Length = 2961

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 21 cacccaatcgtcaaac 36
||||||||||||||||
Sbjct: 1415 cacccaatcgtcaaac 1400

>gi|18121489|emb|AL513022.19| Mouse DNA sequence from clone RP23-3N21 on chromosome 13 Contains the
gene for a novel serine (or cysteine) proteinase
inhibitor, clade B (Serpinb) member, six Serpin
pseudogenes and a pseudogene similar to part of Casitas
B-lineage lymphoma (Cbl), complete sequence
Length = 208922

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 14 caaagcccacccaatc 29
||||||||||||||||
Sbjct: 29726 caaagcccacccaatc 29741

>gi|20260774|gb|AC025198.12| Homo sapiens chromosome 17, clone RP11-766H1, complete sequence
Length = 198524

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 15 aaagcccacccaatcg 30
||||||||||||||||
Sbjct: 114940 aaagcccacccaatcg 114955

>gi|19849359|gb|AC091549.11| Homo sapiens chromosome 8, clone RP11-343D20, complete sequence
Length = 179187

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 13 gcaaagcccacccaat 28
||||||||||||||||
Sbjct: 91055 gcaaagcccacccaat 91040

>gi|2814534|emb|Z83216.1|CEC08F11 Caenorhabditis elegans cosmid C08F11, complete sequence
Length = 45679

Score = 32.2 bits (16), Expect = 42
Identities = 19/20 (95%)
Strand = Plus / Minus


Query: 16 aagcccacccaatcgtcaaa 35
||||||||| ||||||||||
Sbjct: 13681 aagcccaccaaatcgtcaaa 13662

>gi|11595616|emb|AL451018.1|NC99H12 Neurospora crassa DNA linkage group V Cosmid contig 99H12
Length = 85165

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Minus


Query: 11 tcgcaaagcccaccca 26
||||||||||||||||
Sbjct: 17177 tcgcaaagcccaccca 17162

>gi|12718469|emb|AL513466.1|NCB18D24 Neurospora crassa DNA linkage group V BAC contig B18D24
Length = 157180

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 11 tcgcaaagcccaccca 26
||||||||||||||||
Sbjct: 48064 tcgcaaagcccaccca 48079

>gi|13346588|gb|AC020601.10| Homo sapiens BAC clone RP11-451C14 from 2, complete sequence
Length = 204164

Score = 32.2 bits (16), Expect = 42
Identities = 16/16 (100%)
Strand = Plus / Plus


Query: 7 aaagtcgcaaagccca 22
||||||||||||||||
Sbjct: 46789 aaagtcgcaaagccca 46804



Lambda K H
1.37 0.711 1.31

Gapped
Lambda K H
1.37 0.711 1.31

Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 2795139
Number of Hits to DB: 167,206,298
Number of extensions: 6862408
Number of successful extensions: 4966
Number of sequences better than 1000.0: 190
Number of HSP's better than 1000.0 without gapping: 190
Number of HSP's gapped: 4966
Number of HSP's successfully gapped: 195
Number of extra gapped extensions for HSPs above 1000.0: 4698
Length of query: 36
Length of database: 12,445,000,397
Length adjustment: 19
Effective length of query: 17
Effective length of database: 12,391,892,756
Effective search space: 210662176852
Effective search space used: 210662176852
A: 0
X1: 11 (21.8 bits)
X2: 15 (30.0 bits)
X3: 25 (50.0 bits)
S1: 12 (25.0 bits)
S2: 14 (28.2 bits)
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