『原创』大家在做蛋白时的buffer配方（附件）

Premixed Electrophoresis Buffer Composition
Buffer  1x Formulation  Application(s)
10x Tris/glycine/SDS  25 mM Tris, pH 8.3, 192 mM glycine, 0.1% SDS   SDS-PAGE
10x Tris/glycine  25 mM Tris, pH 8.3, 192 mM glycine   PAGE
10x Tris/Tricine/SDS  100 mM Tris, pH 8.3, 100 mM Tricine, 0.1% SDS   Peptide analysis, small protein SDS-PAGE
10x IEF anode buffer  7 mM phosphoric acid   Analytical IEF
10x IEF cathode buffer  20 mM lysine, 20 mM arginine   Analytical IEF
10x zymogram renaturation buffer  2.5% Triton X-100   Protease analysis; renatures enzymes after electrophoresis
10x zymogram development buffer  50 mM Tris-HCl, pH 7.5, 200 mM NaCl,
5 mM CaCl2, 0.02% Brij-35  Protease analysis; activates enzymes after electrophoresis
Premixed Sample Buffer Composition
Buffer  Formula  Application(s)
Laemmli sample buffer  62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% Bromophenol Blue  SDS-PAGE
Native sample buffer  62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% Bromophenol Blue  PAGE
Tricine sample buffer  200 mM Tris-HCl, pH 6.8, 2% SDS, 40% glycerol, 0.04% Coomassie Blue G-250  Peptide and small protein SDS-PAGE
IEF sample buffer  50% glycerol  Isoelectric focusing
Zymogram sample buffer  62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 4% SDS, 0.01% Bromophenol Blue  Protease analysis