Extraction of RNA from Fibrous tissue
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实验原理
实验试剂
RNase-free Proteinase K (20mg/ml)
实验设备
Water bath or heat block preset at 55°C
实验步骤
2. Dilute the lysate with 590 ul of DEPC Water and vortex to mix well.
5. Add 0.5 volume of absolute ethanol ( room temperature ) to the mixture and mix well by pipetting.
9. DNase I digestion (Optional)
1) For each HiBind® RNA column, prepare the DNase I digestion reaction mix as follows:
OBI DNase I Digestion Buffer 73.5 ul
RNase-free DNase I (20 Kunitz unites/ul) 1.5 ul
b. OBI DNase I digestion buffer is supplied with OBI RNase-free DNase set.
c. Standard DNase buffers are not compatible with on-membrane DNase digestion.