Shan9
文献原文:CD32-expressing murine fibroblast L cells were gifted to D.A.R. by M. Levings. CD32-expressing L cells between passage
numbers 4 and 20 were incubated with an agonist anti-CD3 antibody
(OKT3, Bio X Cell) for 30 min on ice and then plated with
sorted CD8 T cells at a ratio of 5:1 CD8 T cells:L cells.(PMID: 37506196.)
我百思不得其解的两个问题:
1. 这里为什么要用表达CD32的细胞系与CD8共培进行杀伤实验呢?文章对CD32也没有特殊研究目的,CD32的表达也对CD8的杀伤起不到作用吧?如果有的话请大家赐教。
2. anti-CD3 antibody有什么作用呢?文章引用的另一篇文献PMID: 36470208也提到了这个
该问题暂时还没有回答
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