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蛋白棕榈酰化

相关实验:蛋白质的分离纯化

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廿一的坚果

各位园子里的友友们,有对棕榈酰化比较了解,做过相关实验的么?文章中也只是简单的概述,看了看园子里的帖子,也只是原理性描述,对于相关实验的讨论并没有见到,希望有了解的uu能有后分享一些详细的protocol和经验,感激不尽!

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1 个回答

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Dr_劉医生

有帮助

之前听过拜谱的工程相关的讲座,棕榈酰化修饰蛋白组现在也是比较前沿的技术,相信文献你也找到过,我也附上几篇综述;主要因为技术路线都涉及质谱等仪器,导致很多实验操作都很难,可以去咨询相关生物科技公司,外包或者请教他们一下;

参考文献:

[1] Main A, Fuller W. Protein S‐Palmitoylation: Advances and Challenges in Studying a Therapeutically Important Lipid Modification[J]. The FEBS Journal, 2021.

[2] Hornemann T. Palmitoylation and depalmitoylation defects[J]. Journal of Inherited Metabolic Disease, 2015,38(1): 179-186.

[3] Ohn T, Anderson P. The role of posttranslational modifications in the assembly of stress granules[J]. Wiley Interdisciplinary Reviews-RNA 2010,1(3): 486-493.

[4] Zareba-Koziol M, Bartkowiak-Kaczmarek A, Figiel I, et al. Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins[J]. Molecular & Cellular Proteomics, 2019, 18(10): 1916-1938.

[5] Globa A K, Bamji S X. Protein palmitoylation in the development and plasticity of neuronal connections[J]. Current opinion in neurobiology, 2017, 45: 210-220.

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