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CTAB法是一种常用的植物DNA提取方法。以下是该方法实验数据处理方法及参考文献的建议:
实验数据处理方法:
使用比色法、比浊法或荧光分光光度法测定DNA的质量和浓度;
根据DNA的质量和浓度计算出DNA的摩尔浓度;
计算每个样品的DNA纯度,包括260nm/280nm比值和260nm/230nm比值,以评估是否有污染和DNA纯度是否足够高;
可以进行凝胶电泳检测,评估DNA的完整性和是否有RNA或其他污染物;
最后,将提取的DNA保存在-20℃或更低的温度下,以备后续实验使用。
参考文献:
Doyle, J.J. and Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8: 4321-4325.
Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minipreparation: Version II. Plant Molecular Biology Reporter, 1: 19-21.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A. and Allard, R.W. (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America, 81: 8014-8018.
土井挞克树
实验数据处理可以跑完wb或者pcr后看条带或者荧光情况
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