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    SNuPE Protocol

    互联网

    1222

     

    1. Make sure that there is enough dCTP and TTP (or dGTP and dATP) in the hot room
    2. Set up your PCR master mix.  Remember that you need to do a reaction for dCTP and TTP for each sample
      1. 10xPCR Buffer  2.5uL
      2. 0.1M MgCl2  0.375ul
      3. Primer  1ul
      4. Taq  1ul
      5. dH20  - enough to bring final volume to 25 ul

     

     

     

     

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