Genotyping Single Nucleotide Polymorphisms by Multiplex Minisequencing Using Tag-Arrays
互联网
523
The need for multiplexed methods for SNP genotyping has rapidly increased during the last decade. We present here a flexible system that combines highly specific genotyping by minisequencing single-base extension with the advantages of a microarray format that allows highly multiplexed and parallel analysis of any custom selected SNPs.
Cyclic minisequencing reactions with fluorescently labeled dideoxynucleotides (ddNTPs) are performed in solution using multiplex PCR product as template and detection primers, designed to anneal immediately adjacent and upstream of the SNP site. The detection primers carry unique Tag-sequences at their 5′ ends and oligonucleotides complementary to the Tag-sequence, cTags, are immobilized on a microarray. After extension, the tagged detection primers are allowed to hybridize to the cTags, and the fluorescent signals from the array are measured and the genotypes are deduced by cluster analysis of the incorporated labels. The “array of arrays” format of the system, accomplished by a silicon rubber grid to form separate reaction chambers, allows either 80 or 16 samples to be analyzed for up to 200 or 600 SNPs, respectively on a single microscope slide.
