Multiplex ABO Genotyping by Minisequencing

The minisequencing multiplex reaction to genotype ABO blood group system is described and discussed. This method was found to be a reproducible strategy to type 6 common alleles (A1, A2, B, 01, 01 V, and 02) of the ABO blood group system, the high specificity and sensitivity make it suitable also in forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutations are readily investigated by the addition of new extension primers in the minisequencing multiplex reaction.