小鼠单链抗体表达库所设计的VH和VL引物
互联网
4202
小鼠单链抗体表达库所设计的 VH 和 VL 引物
|
|
VH FR1引物
|
VL FR1引物
|
|
1
|
5’-GGCTCGAGCAGGTT(G)CAGCTGC(A)AGC
AGTCT(A)GG
|
5’-CCTCTAGAGACATCCAGATGAC(A)CCAGTCTCC
|
|
2
|
5’-GCTCGAGCAGGTG(A)CAGCTGG(A)T(A)
GC(G)AGTCT(A)GG
|
5’-CCTCTAGAGACATT(C)G(T)TGCTGACC(T)CAA
(G)TCTCC
|
|
3
|
5’-GGCTCGAGCAGGTCCAACTGCAGC(G)AGC
(T)CTGG
|
5’CCTCTAGAG(A)ACATTGTG(A)ATGACA(C)CAG(A)TCTCC
|
|
4
|
5’-GGCTCGAGGAGGTGA(C)AGCTGGTGGAA
(G)TCT(C)GG
|
5’-CCTCTAGAGACATC(T)CAGA(C)TGACT(C)CAGTC
TCC
|
|
5
|
5’-GGCTCGAGGAG(A)GTGAA(T)GC(G)TGG
TGGAGTCTGG
|
5’-CCTCTAGAGGAGACATTGTGATGACCCAGTC
|
|
6
|
5’-GGCTCGAGGAG(A)GTCCAGCTT(G)CA
GCAGTCAGG
|
5’-CCTCTAGAGATATTGTGA(C)TAACC(T)CAGG(T)A
(C)TG(C)A(C)AC(G)T(C)C
|
|
7
|
5’-GGCTCGAGGAGGTCCAGCTGCAA(G)CAGT
CC(A)GG(T)A(G)C(G)C
|
5’-CCTCTAGAGATATCG(C)T(A)T(G)ATGACC(A)CA
GT(A)CTC(A)C
|
|
8
|
5’-GGCTCGAGGAGGTTCAGCTT(G)CAGCAGT
CTGG(T)A(G)GC
|
5’-CCTCTAGAGCTGTTGTGA(G)TGACC(T)CAAACTCC
|
|
9
|
5’-GGCTCGAGGAG(A)GTG(T)AAG(A)CTT
(G)C(G)TC(A)GAGTCTGG
|
5’-CCTCTAGAGATGTTT(G)TGATGACCCAA(C)ACTCC
|
|
10
|
5’-GCTCGAGGATGTGCAGCTG(T)G(C)T(A)
GGAGTCT(A)GG
|
5’-CCTCTAGAGATGTTTTGATGAA(C)CCAAAC(T)TCC
|
|
11
|
5’-GGCTCGAGCAGTCAGTGAAGGAGTCCGA
GGG
|
5’-CCTCTAGAGAA(G)AATG(C)TGCTCACCCAGTCTCC
|
|
12
|
5’-GGCTCGAGGACTACTACATAAACTGGGTGA
AAC
|
5’-CCTCTAGAG(C)AAATTGTTCTCACCCAGTCTCC
|
|
13
|
|
5’-CCTCTAGACAGGCTGTTGTGACTCAGGAATC
|
|
14
|
5’-GGACTAGTT(G)G(A)A(G)G(C)GAGACT
GTGAGAGTGGTG(T)C
|
5’-CCGAATTCTTTG(T)ATTTCCAGCTTGGTG(C)CCT
(C)C
|
|
15
|
5’-GGACTAGTTGC(A)A(G)GAG(A)ACAGT
GACCA(G)G(T)A(G)GTCC
|
5’-CCGAATTCTTTGAT(G)CTCCAGCTTGGTCCCCC
|
|
16
|
5’-GGACTAGTTGAGGAGACG(A)GTGACTG
A(T)GGTTC
|
5’-CCGAATTCTTTT(C)AT(G)T(C)TCCAA(G)CTTT
(G)GTCCCCG
|
|
17
|
5’-GGACTAGTT(A)GC(A)AGAG(A)ACA(G)
GTG(A)AG(C)A(G)GTGGTG(A)C
|
5’-CCGAATTCA(G)CCGACAGTCAGTTTGGTT(A)CCTC
|
|
18
|
5’-GGACTAGTT(C)GAGGAGACGGTGACCG
(A)T(G)GGTC(G)C
|
5’-CCGAATTCA(T)C(A)C(G)GACAGTGACCTT GGTTCCAC
|
|
19
|
|
5’-CCGAATTCAGCGCGCTTCAGTTCCAGTTTGG
|
