小鼠单链抗体表达库所设计的VH和VL引物
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小鼠单链抗体表达库所设计的 VH 和 VL 引物
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VH FR1引物
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VL FR1引物
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1
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5’-GGCTCGAGCAGGTT(G)CAGCTGC(A)AGC
AGTCT(A)GG
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5’-CCTCTAGAGACATCCAGATGAC(A)CCAGTCTCC
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2
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5’-GCTCGAGCAGGTG(A)CAGCTGG(A)T(A)
GC(G)AGTCT(A)GG
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5’-CCTCTAGAGACATT(C)G(T)TGCTGACC(T)CAA
(G)TCTCC
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3
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5’-GGCTCGAGCAGGTCCAACTGCAGC(G)AGC
(T)CTGG
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5’CCTCTAGAG(A)ACATTGTG(A)ATGACA(C)CAG(A)TCTCC
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4
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5’-GGCTCGAGGAGGTGA(C)AGCTGGTGGAA
(G)TCT(C)GG
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5’-CCTCTAGAGACATC(T)CAGA(C)TGACT(C)CAGTC
TCC
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5
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5’-GGCTCGAGGAG(A)GTGAA(T)GC(G)TGG
TGGAGTCTGG
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5’-CCTCTAGAGGAGACATTGTGATGACCCAGTC
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6
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5’-GGCTCGAGGAG(A)GTCCAGCTT(G)CA
GCAGTCAGG
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5’-CCTCTAGAGATATTGTGA(C)TAACC(T)CAGG(T)A
(C)TG(C)A(C)AC(G)T(C)C
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7
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5’-GGCTCGAGGAGGTCCAGCTGCAA(G)CAGT
CC(A)GG(T)A(G)C(G)C
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5’-CCTCTAGAGATATCG(C)T(A)T(G)ATGACC(A)CA
GT(A)CTC(A)C
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8
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5’-GGCTCGAGGAGGTTCAGCTT(G)CAGCAGT
CTGG(T)A(G)GC
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5’-CCTCTAGAGCTGTTGTGA(G)TGACC(T)CAAACTCC
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9
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5’-GGCTCGAGGAG(A)GTG(T)AAG(A)CTT
(G)C(G)TC(A)GAGTCTGG
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5’-CCTCTAGAGATGTTT(G)TGATGACCCAA(C)ACTCC
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10
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5’-GCTCGAGGATGTGCAGCTG(T)G(C)T(A)
GGAGTCT(A)GG
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5’-CCTCTAGAGATGTTTTGATGAA(C)CCAAAC(T)TCC
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11
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5’-GGCTCGAGCAGTCAGTGAAGGAGTCCGA
GGG
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5’-CCTCTAGAGAA(G)AATG(C)TGCTCACCCAGTCTCC
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12
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5’-GGCTCGAGGACTACTACATAAACTGGGTGA
AAC
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5’-CCTCTAGAG(C)AAATTGTTCTCACCCAGTCTCC
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13
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5’-CCTCTAGACAGGCTGTTGTGACTCAGGAATC
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14
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5’-GGACTAGTT(G)G(A)A(G)G(C)GAGACT
GTGAGAGTGGTG(T)C
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5’-CCGAATTCTTTG(T)ATTTCCAGCTTGGTG(C)CCT
(C)C
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15
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5’-GGACTAGTTGC(A)A(G)GAG(A)ACAGT
GACCA(G)G(T)A(G)GTCC
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5’-CCGAATTCTTTGAT(G)CTCCAGCTTGGTCCCCC
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16
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5’-GGACTAGTTGAGGAGACG(A)GTGACTG
A(T)GGTTC
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5’-CCGAATTCTTTT(C)AT(G)T(C)TCCAA(G)CTTT
(G)GTCCCCG
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17
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5’-GGACTAGTT(A)GC(A)AGAG(A)ACA(G)
GTG(A)AG(C)A(G)GTGGTG(A)C
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5’-CCGAATTCA(G)CCGACAGTCAGTTTGGTT(A)CCTC
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18
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5’-GGACTAGTT(C)GAGGAGACGGTGACCG
(A)T(G)GGTC(G)C
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5’-CCGAATTCA(T)C(A)C(G)GACAGTGACCTT GGTTCCAC
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19
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5’-CCGAATTCAGCGCGCTTCAGTTCCAGTTTGG
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