【讨论】可以检测细胞内部的分泌型蛋白吗?
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检测细胞的VEGF表达量,因为是分泌型蛋白,成熟体都分泌在胞外,大部分文献都是取细胞培养液上清做Elisa。
如果实验要求不能检测上清液,可否用Elisa检测细胞内部的VEGF表达?
胞内胞外的VEGF表达量是平行的,还是相对的?
如果实验要求不能检测上清液,可否用Elisa检测细胞内部的VEGF表达?
胞内胞外的VEGF表达量是平行的,还是相对的?
谢谢斑竹!
那么是不是内部表达的VEGF高了,就说明细胞分泌在胞外的也高?二者的量是平行的吗?
那么是不是内部表达的VEGF高了,就说明细胞分泌在胞外的也高?二者的量是平行的吗?
自己顶~~~
期待做过胞内VEGF检测的高手指点一二啊~~~
期待做过胞内VEGF检测的高手指点一二啊~~~
stillhorse wrote:
自己顶~~~
期待做过胞内VEGF检测的高手指点一二啊~~~
你自己要学会检索啊。我用“intracellular VEGF detection”在google.com里搜索了一下,第四个结果就有用ELISA检测胞内VEGF的报道。
http://www.nature.com/leu/journal/v17/n10/full/2403084a.html
For intracellular levels of VEGF, 5x 106 cells from each cell line were washed in RPMI x2, snap frozen in liquid nitrogen, and then thawed at 37°C. The snap freezing and thawing was repeated two more times. Cellular debris was then spun down and supernatant saved for analysis.
另外:
miR-20b modulates VEGF expression by targeting HIF-1α and STAT3 in MCF-7 breast cancer cells†。Journal of Cellular Physiology,Volume 224, Issue 1, pages 242–249, July 2010
freecell wrote:
你自己要学会检索啊。我用“intracellular VEGF detection”在google.com里搜索了一下,第四个结果就有用ELISA检测胞内VEGF的报道。
http://www.nature.com/leu/journal/v17/n10/full/2403084a.html
For intracellular levels of VEGF, 5x 106 cells from each cell line were washed in RPMI x2, snap frozen in liquid nitrogen, and then thawed at 37°C. The snap freezing and thawing was repeated two more times. Cellular debris was then spun down and supernatant saved for analysis.
另外:
miR-20b modulates VEGF expression by targeting HIF-1α and STAT3 in MCF-7 breast cancer cells†。Journal of Cellular Physiology,Volume 224, Issue 1, pages 242–249, July 2010
非常感谢斑竹!
我也搜了,关键词没搞对~~
再次感谢!
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