Linear Amplification Sequencing with Dye Terminators
互联网
449
Present day sequencing technology is mainly based on the dideoxy method introduced by Sanger in 1977 (1 ;see ). DNA sequencing is a complex process requiring many different complicated steps like subcloning, template preparation, sequencing reactions, gel electrophoresis, reading the nucleotide sequence into the computer, and data handling. Various modifications may involve synthetic primers, enzymatic manipulations, and polymerase chain reactions
