Agrobacterium-Mediated Transformation of Petunia Leaf Disks

Agrobacterium -mediated transformation of plants is now applicable to many dicotyledonous and also several monocotyledonous plant species. It can be used to transform many different species based on various factors: the broad host range of Agrobacterium (1 ), the regeneration responsiveness of many different explant tissues (2 ), and the utility of a wide range of selectable marker genes (3 ). In addition to tobacco, one of the first species that was routinely transformed using this method was Petunia hybrida .