丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Agrobacterium-Mediated Transformation of Cotton

互联网

743
There are many methods and techniques that can be used to transfer foreign genes into cells. In plant biotechnology, Agrobacterium -mediated transformation is a widely used traditional method for inserting foreign genes into plant genome and obtaining transgenic plants, particularly for dicot plant species. Agrobacterium -mediated transformation of cotton involves several important and also critical steps, which includes coculture of cotton explants with Agrobacterium , induction and selection of stable transgenic cell lines, recovery of plants from transgenic cells majorly through somatic embryogenesis, and detection and expression analysis of transgenic plants. In this chapter, we describe a detailed step-by-step protocol for obtaining transgenic cotton plants via Agrobacterium -mediated transformation.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序