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Agrobacterium-Mediated Transformation of Petunia Leaf Discs

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Many dicotyledonous and also several monocotyledonous plant species are susceptible to Agrobacterium -mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an Agrobacterium -mediated transformation method of Petunia hybrida leaf discs, one of the first species that was routinely transformed using this method.
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