丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Brandford法测定蛋白浓度

互联网

8341

Bradford quantification of protein concentration Brandford法测定蛋白浓度

Standard Curve

<center>m g of standard BSA</center>

<center>0.2 mg/mL BSA stock</center><center>(m L)</center>

<center>dH2 O (m L)</center>

<center>Bradford mix (m L)</center>

1

5

795

200

2

10

790

200

4

20

780

200

6

30

770

200

8

40

760

200

Wait for 5 minutes and then read absorbance at 595nm

At the same time,prepare your samples of interest as above and meaure absorbance after measuring the standards.Take an average of the standard sample and determine the absorbance that defines 1 mg of BSA and use that to measure the concentration of the unknown samples.

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序