Construction of Linker-Scanning Mutations Using the Polymerase Chain Reaction
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In the previous chapter we described linker scanning mutagenesis by oligonucleotide ligation. In this chapter we describe a more recent and versatile procedure that makes use of amplification by the polymerase chain reaction (PCR). For a description of the traditional method of generating linker scanning mutants by combination of deletion fragments, see ref. 1 .
