Construction of Linker-Scanning Mutations by Oligonucleotide Ligation
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1. | The approach is systematic and implies no previous prejudice about the location of functional elements; |
2. | The same mutated segment is substituted in each mutant, so that potential contributions to activity from the mutated segment are minimized when comparing mutants; and |
3. | Most importantly, the spacing and torsional orientation of remaining functional elements remains unchanged in the mutants. |