Polymerase Chain Reaction
互联网
835
<center>
<p>
</p>
</center>
-
上一篇:PCR Amplification of DNA 下一篇:Standard PCR Protocol
-
-
PCR enzyme (5 Units/ul)
- PCR enzyme reaction buffer
- Distilled water (nuclease free)
- DNA to be used as template, such as GAPDH template from Clontech
- DNA to be used as upstream and downstream primers, such as the corresponding primers set for GAPDH template from Clontech
- Light mineral oil (nuclease free)
-
dNTP mix (10 mM of each dNTP)
-
-
PCR machine and the 600-ul tubes for use in the machine
- Micropipetter and tips
- Microcentrifuge with adaptors for 600-ul tubes
-
-
dNTP mix (10 mM of each dNTP)
-
-
PCR machine and the 600-ul tubes for use in the machine
- Micropipetter and tips
- Microcentrifuge with adaptors for 600-ul tubes
-