【求助】紧急求助!!!microRNA基因序列问题
丁香园论坛
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1)我得到的pre-miR-10a系列如下:1 gatctgtctg tcttctgtat ataccctgta gatccgaatt tgtgtaagga attttgtggt 61 cacaaattcg tatctagggg aatatgtagt tgacataaac actccgctct
2)在NCBI Blast得到pre-miR-10a及其上下游各100bp序列如下:
1 taaagaggaa ggagtcttcg tgtggccagt caccagactg tcctcattca gaaaaatact 61 acatatacat atatatatat ttttttcttc tggaaaaata agagcggagt gtttatgtca 121 actacatatt cccctagata cgaatttgtg accacaaaat tccttacaca aattcggatc 181 tacagggtat atacagaaga cagacagatc ttcttggccc agaaattttc cagttctcct 241 actttccgcg ccttcttcgt ttggggtgtg gtttgagtgc tttggtttta tttcgttttg 301 ttctagtttt
现非常疑惑为什么2)中的序列中没有包括1)中的序列,非常感谢各位的帮助!!!!!!
2)在NCBI Blast得到pre-miR-10a及其上下游各100bp序列如下:
1 taaagaggaa ggagtcttcg tgtggccagt caccagactg tcctcattca gaaaaatact 61 acatatacat atatatatat ttttttcttc tggaaaaata agagcggagt gtttatgtca 121 actacatatt cccctagata cgaatttgtg accacaaaat tccttacaca aattcggatc 181 tacagggtat atacagaaga cagacagatc ttcttggccc agaaattttc cagttctcct 241 actttccgcg ccttcttcgt ttggggtgtg gtttgagtgc tttggtttta tttcgttttg 301 ttctagtttt
现非常疑惑为什么2)中的序列中没有包括1)中的序列,非常感谢各位的帮助!!!!!!
hsa-mir-10a gene sequence with 100 flank:
AAAACTAGAACAAAACGAAATAAAACCAAAGCACTCAAACCACACCCCAAACGAAGAAGGCGCGGAAAGTAGGAGAACTGGAAAATTTCTGGGCCAAGAAGATCTGTCTGTCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAAGGAATTTTGTGGTCACAAATTCGTATCTAGGGGAATATGTAGTTGACATAAACACTCCGCTCTTATTTTTCCAGAAGAAAAAAATATATATATATGTATATGTAGTATTTTTCTGAATGAGGACAGTCTGGTGACTGGCCACACGAAGACTCCTTCCTCTTTA
pre-hsa-mir-10a sequence
GATCTGTCTGTCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAAGGAATTTTGTGGT
不知道你找的是什么物种的miRNA,给你找了个人的。数据来源:
http://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000207777;r=17:46657200-46657309;t=ENST00000385043
AAAACTAGAACAAAACGAAATAAAACCAAAGCACTCAAACCACACCCCAAACGAAGAAGGCGCGGAAAGTAGGAGAACTGGAAAATTTCTGGGCCAAGAAGATCTGTCTGTCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAAGGAATTTTGTGGTCACAAATTCGTATCTAGGGGAATATGTAGTTGACATAAACACTCCGCTCTTATTTTTCCAGAAGAAAAAAATATATATATATGTATATGTAGTATTTTTCTGAATGAGGACAGTCTGGTGACTGGCCACACGAAGACTCCTTCCTCTTTA
pre-hsa-mir-10a sequence
GATCTGTCTGTCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAAGGAATTTTGTGGT
不知道你找的是什么物种的miRNA,给你找了个人的。数据来源:
http://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000207777;r=17:46657200-46657309;t=ENST00000385043
AAAACTAGAACAAAACGAAATAAAACCAAAGCACTCA
AACCACACCCCAAACGAAGAAGGCGCGGAAAGTAGGA
GAACTGGAAAATTTCTGGGCCAAGAAGATCTGTCTG
TCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAA
GGAATTTTGTGGTCACAAATTCGTATCTAGGGGAATA
TGTAGTTGACATAAACACTCCGCTCTTATTTTTCCAG
AAGAAAAAAATATATATATATGTATATGTAGTATTTT
TCTGAATGAGGACAGTCTGGTGACTGGCCACACGAA
GACTCCTTCCTCTTTA
刚才的序列没有显示完全,补上,也可以点击链接打开数据库自己下载。
AACCACACCCCAAACGAAGAAGGCGCGGAAAGTAGGA
GAACTGGAAAATTTCTGGGCCAAGAAGATCTGTCTG
TCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAA
GGAATTTTGTGGTCACAAATTCGTATCTAGGGGAATA
TGTAGTTGACATAAACACTCCGCTCTTATTTTTCCAG
AAGAAAAAAATATATATATATGTATATGTAGTATTTT
TCTGAATGAGGACAGTCTGGTGACTGGCCACACGAA
GACTCCTTCCTCTTTA
刚才的序列没有显示完全,补上,也可以点击链接打开数据库自己下载。
非常感谢您的帮助!
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