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测序通用引物

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相关专题
引物设计
载体 名称上游引物 ( 5'primer )下游引物( 3'primer )
PundefinedFLAG-CMVCMV-30(825-854)CMV-24(1129-1152)
pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH rev
pAcG2TpGEX-5not available
pACTT7EEVT3/EBVrev
pACT2GAL4 ADforpGAL4-ADrv
pACT2p17110p12584
pACYC184(BamHI-site)PBRforBamPBRrevBam
pACYCDuet-1(MCSI)ACYCDuetUP1 PrimerDuetDOWN1
pACYCDuet-1(MCSII)DuetUp2T7-Terminator
pAdEasy-1not availablenot available
pAdTrack-CMVnot availablenot available
pAS2-1GAL4 Bdfor/P24990pGAL4-BDrv/P31430
pB42ADpB42ADFPB42ADR
pBABEpBMN_5' 
pBacPAK8BAC 1BAC2
pBADpBAD-FpBAD-R
pBAD/HisABCpBAD-5' 
pBBR1MCST3/M13revT7/M13for
pBD-GAL4pBD-GAL4-FPBD-GAL4-R
pBI12135SNOS
pBINDGAL4-BdforT3,EBVrev
pBK-CMVM13F/T7M13R/T3
pBluescriptIIKS(+/-)T3/M13revT7/M13for(优先用M13F)
pBluescriptIISK(+/-)T3/M13revT7/M13for
pBluescriptKS(+/-)T3/M13revT7/M13for
pBluescriptSK(+/-)T3/M13revT7/M13for
pBR322(BamHI-site)PBRforBamPBRrevBam
pBR322(EcoRI/HindIII-sites)pBRforEcopBRrevHind
pBR325(BamHI-site)PBRforBamPBRrevBam
pBR329(BamHI-site)PBRforBamPBRrevBam
pBridge(MCSI)GAL4-Bdfor3'BD
pBridge(MCSII)not availablenot available
pBS-T IIT3/M13revT7/M13for
pBS(AMP)T7T3
pBSRT3T7ter
pBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrev
pBudCE4.1(MCSII)EF-1α FwdBGH
pBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrev
pBV220pBV220FPBV220R
pCAL-cT7/Tac-ProforT7-Terminator
pCAL-nT7/Tac-ProforT7-Terminator
pCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)
pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)
pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)
pCANTB5ES1S6
pCAT3-enhancerRVP3此载体无反向引物
pCDFDuet-1(MCSI)ACYCDuetUP1 PrimerDuetDOWN1
pCDFDuet-1(MCSII)DuetUP2T7TER
pcDNA1.1CMV-Profor,T7pCDM8-3
pcDNA2.1M13for/T7M13rev/Tac-Profor
pcDNA3T7/CMV-ProforBGH rev/SP6
pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGH rev
pcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGH rev
pcDNA3.1/myc-His(_A,_B,_C)T7/CMV-ProforBGH rev/c-mycrev
pcDNA3.1/V5-His(_A,_B,_C)T7/CMV-ProforBGH rev/V5rev
pcDNA3.1/Hygro(+,-)T7/CMV-ProforBGH rev/V5rev
pcDNA3.1/Zeo(+.-)T7BGHrev
pcDNA3.3TOPOTACMV-ProforTKPAreverse
pcDNA4/HisMax(_A,_B,_C)XpressforBGH rev
pcDNA4/HisMax-TOPOXpressforBGH rev
pcDNA4/HisMax-TOPO/lacZXpressforBGH rev
pcDNA4/myc-His(_A,_B,_C)T7,CMV-ProforBGH rev,c-mycrev
pcDNA4/TOTREfor/CMV-ProforBGH rev
pcDNA4/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5rev
pcDNA4-TET/ON/AMP+CMV-Profor 
pcDNA5/FRTT7/CMV-ProforBGH rev
pcDNA6/myc-His(_A,_B,_C)CMV-Profor,T7BGH rev,c-mycrev
pcDNA6/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5rev
pCEP4pCEP-F/CMV-ProforEBVrev
pCIT7(17BASE)EBVrev
pCI-neoT7(17BASE)EBVrev,T3
pCMS-EGFPT7T3
pCMS-EGFPT7EBVrev,T3
pCMV-3Tag-4AT3 /PFLAG-CMV-FT7
pCMV5pCMV5FPCMV5R
pCMV6CMV-ProforpCMV6-3、HGHrev
pCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrev
pCMV-HAPCMV-F,CMV-Profor,TREforPCMV-R,EBVrev
pCMV-MYC(AMP+)PCMV-FPCMV-R, EBVrev
pCMVmyc/nucCMV-ProforBGH rev,c-mycrev
pcmv-scriptEXVectorT3T7
pCMV-Sport6T7/M13forSP6/M13rev
pCOLADuet-1(MCSI)ACYCDuetUP1 PrimerDuetDOWN1
pCOLADuet-1(MCSII)DuetUP2T7TER
pCR2.1T7,M13forM13rev
pCR2.1-TOPOM13for,T7M13rev
pCR3.1T7BGH rev
pCR3.1-UniT7BGH rev
pCR4T3/M13revT7/M13for
pCR4-BluntT3/M13revT7/M13for
pCR4-TOPOM13rev,T3M13for,T7
pCR-BluntM13revT7,M13for
pCR-BluntIIM13rev/SP6T7/M13for
pCR-BluntII-TOPOM13rev/SP6M13for/T7
pCRIIM13rev/SP6T7/M13for
pCRII-TOPOM13rev/SP6M13for/T7
pCR-ScriptSK(+)T7/M13forT3/M13rev
pCR-XL-TOPOM13revT7,M13for
pCS2+(MCSI)SP6EBVrev,T7?
pCS2+(MCSII)T3pSG5-3
pDB_GAL4camT7P 
pDB_leuPDBLeu-5PDBLeu-3
pDEST22DEST22FDEST22R
pDEST15pGEX-5T7-Terminator
pDEST20pGEX-5pFastBac-3
pDEST26TREfor/CMV-ProforT7/M13for
pDisplayT7/CMV-Proforc-mycrev
pDNR-LIB(MCS_A)M13for,T7pSG5-3,M13R
pDonarM13FM13R
pDONR201PDONR-FPDONR-R
pDONR207PDONR-FPDONR-R
pDONR223M13FM13R
pDriveT7/M13revSP6/M13for
pDsRED1-C1(DsRed1-N-f)DsRed1-C-r
pDsRed1-N1CMV-ProforRFP-Nrev,CMV-R?
pDsRed2-N1(DsRed1-N-f)DsRed1-C-r
pDsRed2-C1(DsRed1-N-f)DsRed1-C-r
pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3’
pDsRed-ExpressdsRed1_N_primer/CMV-FdsRed1_C_primer/CMV-R
pDSRED-monomer-N1(KAN+)dsRed1_N_primer 
pDSRED-N1(KAN+)pEGFP-N-5’pDSRED-N-R
pEASY-BluntM13FT7/ M13R
pEASY-BluntSimpleM13FT7/ M13R(距离插入位点合适,优先安排)
pEASY-T1 SimpleM13FM13R(距离插入位点合适,优先安排)
pEASY-T1M13F/T7M13R
pEASY-T3M13F/T7M13R/SP6
pECFP-N(C)PECFP-N-5.PECFP-N-3,
pEF/myc/cyto/ER/mito/nucpEF-5Pcdna3.1R/BGH rev
pEF1(4,6/myc-His)T7/pEF-5Pcdna3.1R/BGH rev
pEF1(4,6/V5-His)T7/pEF-5Pcdna3.1R/BGH rev
pEF1(4;6/His_A;_B;_C)T7/XpressforPcdna3.1R/BGH rev
pEF5/FRT/V5-D(-TOPO)T7/pEF-5Pcdna3.1R/BGH rev
pEGFPM13revEGFP-Nrev
pEGFP-1not availableEGFP-Nrev/ PEGFP-N-3′
pEGFP-CPEGFP-C-5′PEGFP-C-3′
pEGFP-C1EGFP-CforSV40-pArev
pEGFP-C2EGFP-CforSV40-pArev
pEGFP-C3EGFP-CforSV40-pArev
pEGFP-FEGFP-CforSV40-pArev
pEGFP-NPEGFP-N-5′PEGFP-N-3′
pEGFP-N1CMV-ProforEGFP-Nrev
pEGFP-N2CMV-ProforEGFP-Nrev
pEGFP-N3CMV-ProforEGFP-Nrev
pENTRM13FM13R
pET-11(-a,-b,-c,-d)T7T7-Terminator
pET-12(-a,-b,-c)T7T7-Terminator
pET-14bT7T7-Terminator
pET-15bT7T7-Terminator
pET-16bT7T7-Terminator
pET-17bT7T7-Terminator
pET-17xbT7T7-Terminator
pET-19bT7T7-Terminator
pET-20b(+)T7T7-Terminator
pET-21(-a,-b,-c,-d)(+)T7T7-Terminator
pET-22b(+)T7T7-Terminator
pET-23(-a,-b,-c,-d)(+)T7T7-Terminator
pET-24(-a,-b,-c,-d)(+)T7T7-Terminator
pET-25b(+)T7T7-Terminator
pET-26b(+)T7T7-Terminator
pET-27b(+)T7T7-Terminator
pET-28a(-b,-c)(+)T7T7-Terminator
pET-29a(-b,-c)(+)S.tag/T7T7-Terminator
pET-3(-a,-b,-c,-d)T7T7-Terminator
pET-30_Ek/LICT7T7-Terminator
pET-30_Xa/LICT7T7-Terminator
pET-30a(-b,-c)(+)S.tag/T7T7-Terminator
pET-31b(+)not availableT7-Terminator
pET-32_Ek/LICTRXforT7-Terminator
pET-32_XA/LICTRXforT7-Terminator
pET-32a(-b,-c)(+)S.tag/TRXforT7-Terminator
pET-33b(+)T7T7-Terminator
pET-34b(+)S.tagT7-Terminator
pET-35b(+)S.tagT7-Terminator
pET-36b(+)S.tagT7-Terminator
pET-37b(+)S.tagT7-Terminator
pET-38b(+)not availableT7-Terminator
pET-39b(+)S.tagT7-Terminator
pET-40b(+)S.tagT7-Terminator
pET-41_Ek/LICGST-CforT7-Terminator
pET-41a(-b,-c)(+)S.tag/pGEX-5T7-Terminator
pET-42a(-b,-c)(+)S.tag/pGEX-5T7-Terminator
pET-43.1a(-b,-c)(+)S.tagcoliDOWN
pET-43.1a_EK/LICS.tagnot available
pET-44a(-b,-c)(+)S.tagT7-Terminator
pET-44a_EK/LICS.tagnot available
pET-45b(+)T7T7-Terminator
pET-46_Ek/LICT7T7-Terminator
pET-5(-a,-b,-c)T7T7-Terminator
pET-52b(+)T7T7-Terminator
pET-7T7T7-Terminator
pET-9(-a,-b,-c,-d)T7T7-Terminator
pETcoco-1T7T7-Terminator
pETDuet-1(MCSI)pETUP1DuetDOWN1
pETDuet-1(MCSII)DuetUp2T7-Terminator
pET-T4T7T7-Terminator
pEYFP-C1pEGFP-C-5’pEGFP-C-3'
pEYFP-N1pEGFP-N-5’,CMV-ProforpEGFP-N-3’
pFLAG-ATSN26forpTrcHis-rv;C24rev
pFLAG-CTCN26forpTrcHis-rv;C24rev
pFLAG1N26forpTrcHis-rv;C24rev
pFLAG-CMV(-2)CMV-30;CMV-ProforCMV-24
pFLAG-CMV1CMV-30CMV-24
pGAD424GAL4_ADfor3'AD
pGADGHGAL4_ADfor3'AD
pGADT7T7/GAL4_ADfor3'AD
pGADT7-RecT7/GAL4_ADfor3'AD
pGADT-TT73,BD
pGAD10GAL4_ADfor3'AD
pGAPZa-Aa-FACTOR3'AOX
pGBKT7T73,BD
pGBT9MATCHMAKER5'DNA-BD/GAL4BDMATCHMAKER3'DNA-BD
pGEM-1T7SP6
pGEM-3T7SP6
pGEM-3Zf(+)(-)SP6/M13revT7/M13for
pGEM-4T7SP6
pGEM-4ZT7/M13forSP6/M13rev
pGEM-5Zf(+)(-)SP6/M13revT7/M13for
pGEM-7Zf(+)(-)SP6/M13revT7/M13for
pGEM-9Zf(+)(-)T7/M13forSP6/M13rev
pGEM-TT7/M13forSP6/M13rev
pGEM-T_EasyT7/M13forSP6/M13rev
pGene/V5-His_A(_B,_C)pGENE-5BGH rev,V5rev
pGEX-2TpGEX-5pGEX-3
pGEX-2TKpGEX-5pGEX-3
pGEX-3XpGEX-5pGEX-3
pGEX-4T-1(-2,-3)pGEX-5pGEX-3
pGEX-5X-1(-2,-3)pGEX-5pGEX-3
pGEX-6P-1(-2,-3)pGEX-5pGEX-3
pGL2GLP1GLP2
pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)
pGL3-Basic(MCSI)RVP3(PGL3+)?GLP2(PGL3-)?
pGL3-Basic(MCSII)RVP4,PLXSN-3'?
pGL4  
pGlow(-TOPO)T7pGlow-3
pGM-TT7SP6
pGWCSP6T7
pHook-2T7/CMV-Profornot available
pIND/V5-His_A(_B,_C)not availableBGH rev,V5rev
PinpointTMVectorPinpointPrimerSP6
pIRES(MCSI)T7pIRES-3
pIRES(MCSII)not availableT3,EBVrev
pIRES2-EGFPCMV-Profor,pIRES2-EGFP.P5’IRESrev,pIRES2-EGFP.P3’
pIRES-hrGFP-2aCMV-Profor,T3pIRES-hrGFP-3
pIRESneo2T7/CMV-ProforpIRES-3
pIVEX(all)T7/pBRrevBamT7-Terminator
pLEXApLEXA-FpLEXA-R
pLNCXpLNCX-FpLNCX-R
pLNCX2CMV-ProforpRevTRE-3
pLVTHM(H1启动子下游)H1primerSP6
pLXSNpLXSN-FpLXSN-R
pMALcMalE primerM13F(-47)
pMAL-c2EMalE primerM13F(-47)
pMAL-C2xPmal-c2x-F(P5’)Pmal-C2X-R(P3’ )
pMALdMalE primerM13F(-47)
pMALeMalE primerM13F(-47)
pMAL-P4XMalE primerM13F(-47)
pMAL-p2XMalE primerM13F(-47)
pMET A/B/CAUG1FAUG1R
pMD18-TM13R(-48)ECORIM13F(-47)(HINDⅢ)
pMD19-TM13F47M13R48
pMD20-TM13F47M13R48
pMOSBlueT7/M13revM13for
pMSCV-puropLXSNfwpMSCVrev
pMT/V5-His_A(_B,_C)MtforBGH rev,V5rev
pNTAPT3T7
pCTAPpCTAP5'primerT7
pOTB7M13rev/T7M13for/SP6
pOptiVEC-TOPO TACMV-F/CMV-ProforEMCVIRES reverse
pPC86PPC86-FPPC86-R
pPC97PBD-GAL4-FPBD-GAL4-R
pPIC3.5K5'AOX3'AOX
pPic9k(A+,Z+)5’AOX(Ppic9k-f)/a-factor3’AOX(Ppic9k-R)
pPICZa5'AOX/PPICZa-F3'AOX
pPROEXHTAM13R(-48)无反向引物
pProEX-HTa(b,c)M13R48/ Tac-ProforpBADrv/pTrcHis-3
pPD129.36 M13F20/M13F
pQE30or40or60(AMP+)PQE30-FPQE-30R
pRC/CMV2/CATCMV/T7 
pRECEIVERCMV-F 此载体无反向引物
pRECEIVER-M03pRECEIVER-M03FpRECEIVER-M03R
pREP4pREP4 forward primerEBV reverse primer
pRK5SP6pSG5-3
pRSETT7/XpressforT7ter
pRSFDuet-1(MCSI)DuetUP1DuetDOWN1
pRSFDuet-1(MCSII)DuetUP2T7TER
pSecTac2T7/CMV-ProforBGH rev/c-mycrev
pSG5pSG5-5,/T7PSG5-R(SV40)
pSHlox1T7SP6
pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-R
pSIT7EBVrev
pSilencer1.0-U6T7T3
pSilencer2.0-U6T7M13for
pSilencer2.1-U6neoT7M13for
pSilencer3.1-H1hygroM13F(-47)3.0REV
pSilencer4_1-CMVneopSilener4.1-5'CMV-Profor
pSILENCEV2.0-U6T72.0REV
pSIneoT7EBVrev,T3
PsitT7T7T
pSK01-TM13FM13R
pSK-CMV(KAN+)T7T3
pSOSPsos5'Psos3'
pSP64SP6M13rev
pSP64/65SP6此载体无反向引物
pSP65SP6M13rev
pSP70SP6T7
pSP71SP6T7
pSP72SP6T7
pSP73SP6T7
pSport1SP6/M13F(-47)T7/M13R
pSPORT1SP6/M13forT7/M13rev
pSPT18SP6T7
pSTBlueT7/M13revSP6/M13for
pSUPER.BasicT7/M13FM13R
pSUPERT7T3
pSURE-TM13FM13R
pSURE-TsimpleM13F-47M13R-48
pSuperCospBRforEcoT7
pT3T7M13rev/T3M13for/T7
pT7-7T7not available
pT7Blue®T7M13F(-47)
pT7T3_18UM13rev/T7M13for/T3
pT7T3D-PACM13rev/T7M13for/T3
pTA2M13F/T7(优先使用M13F)T3/M13R
pT-AdvM13revT7,M13for
pTAgT7/M13revM13for
pTARGETTMpTarget.F(在T7前面)/T7pTarget.R
pTG19-TM13F(-47)M13R(-48)
pTARGETTMT7/M13FPTARGET-SEQ
pThioHisA,B,andCTrx ForwardTrx Reverse / M13for
pTO-T7T7T7TER
pTRC99APTRC99A-FpTrcHis-rv
pTrc99a-cPTRC99C-FPTRC99C-R
pTRC99a-cPTRC99C-FPTRC99C-R
pTrcHisBPTrcHisA-F/XpressforPTrcHisA-R
pTrcHisAPTrcHisA-FPTrcHisA-R
pT-REX-DEST30TREforT7
pTriEx1T7/TriExupTriExdown
pTriEx1.1T7/TriExupTriExdown
pTriEx1.1_HygroT7/TriExupnot available
pTriEx1.1_NeoT7/TriExupIRESrev
pTriplEX1TriplEx-LD-5T7,TriplEx-LD-3
pTriplEX2TriplEx-LD-5;pTR5‘T7,TriplEx-LD-3
pTWIN-1T7T7TER
pTXB1T7MxeInteinrev
pTXB2T7MxeInteinrev
pTXB3T7MxeInteinrev
pTYB1T7Inteinrev
pTYB2T7Inteinrev
pTYB3T7Inteinrev
pTYB4T7Inteinrev
pTZ_18UT7/M13revM13for
pUB6/V5-HisUB-FBGH rev,V5rev
pUC13M13rev/M13rev(-48)M13for/M13for(-49)
pUC18M13rev/M13rev(-48)M13for/M13for(-49)
pUC18(19)/118(119)M13F(-47)M13R(-48)/PUC19(HINDⅢ)
pUC19M13rev/M13rev(-48)M13for/M13for(-49)
pUC57M13rev/M13rev(-48)M13for/M13for(-49)
pUC8M13rev/M13rev(-48)M13for/M13for(-49)
pUC9M13rev/M13rev(-48)M13for/M13for(-49)
pUM-TM13F(-20)M13R(-20)
pUCm-T(AMP+)M13F/T7M13R
pUNI/V5-Hisnot availableBGH rev,V5rev
pVAX1T7T7/CMV-ProforPCDNA3.1R/BGHR
pVP22/mycHisVP22-CforBGH rev,c-mycrev
pVP22/mycHis2T7/CMV-ProforVP22-Nrev
pWE15pBRrevHindT7
pYES2T7pYes2.R
pYES-DEST52T7/GAL1-ProforV5rev/CYC1-Terminator
pYEX_4T_1ClontechpYEX_4T-3
pYEX_4T_2pGEX-5pYEX_4T-4
pYEX_4T_3pGEX-5pYEX_4T-4
pYEX_4T_4pGEX-5pYEX_4T-4
pZeoSV2+/hu-TyrT7not available
pZERO-1M13rev/SP6T7/M13for
pZERO-2M13rev/SP6M13for/T7
pZome-1-CZome-FZome-R
pZome-1-NZome-FZome-R
YCp50(BamHI-site)PBRforBamPBRrevBam
YEp13(BamHI-site)PBRforBamPBRrevBam
YEp24(BamHI-site)PBRforBamPBRrevBam
YIp5(BamHI-site)PBRforBamPBRrevBam
RNAi -ReadypSIREN-RetroQ-U6ZsGreenU6 
菌PCDBT7BGH
天为时代TA载体T7T3
m13(+)T7T3
pMSCV-neopMSCV-5'pMSCV-3'

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