通用引物序列
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| M13 Forward(-20) | 5’d[GTAAAACGACGGCCAG]3’ |
| M13 Forward(-40) | 5’d[GTTTTCCCAGTCACGAC]3’ |
| M13 Reverse | 5’d[CAGGAAACAGCTATGAC]3’ |
| T7 Promoter | 5’d[TAATACGACTCACTATAGGG]3’ |
| T3 Promoter | 5’d[ATTAACCCTCACTAAAGGGA]3’ |
| Sp6 Promoter | 5’d[GATTTAGGTGACACTATAG]3’ |
| M13/pUC Reverse | 5’d[AGCGGATAACAATTTCACACAAGG]3’ |
| M13/pUC Forward | 5’d[CCCAGTCACGACGTTGTAAAACG]3’ |
上一篇：如何设计引物 下一篇：实时PCR/RT-PCR解决方案
