丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Assay of Phospholipase D Activity in Cell-Free Systems

互联网

666
Phospholipase D (PLD) enzymes are present in all animal and plant species and have been linked to many critical cellular processes, including proliferation, differentiation, motility, and secretion. The functional significance of PLD derives from its generation of phosphatidic acid, which has both direct signaling properties via activation of numerous kinases, phosphatases, phopspholipases, and other enzymes, as well as via its conversion to diglycerides, the endogenous activators of protein kinase C. The two mammalian PLD isoforms, PLD1 and PLD2, are peripheral membrane proteins that exhibit important physical and functional interactions with the actin cytoskeleton. We outline a cell-free system for the characterization of mammalian PLDs and their activation by physiologic stimuli or pharmacologic agonists for guanine triphosphate-binding proteins. This assay system is used to illustrate the interactions of PLD1 with specific membrane domains and their associated filamentous and monomeric actin components.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序